G-Sep™ Size Exclusion Column

Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. 

G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. The Columns are available in 16/60 format with two separation range, 3 kDa to 70 KDa and 6 kDa to 600 kDa.

G-Sep™ Size Exclusion Columns, 3-70 kDa have performace similar to Superdex™ 75 pg columns and G-Sep™ Size Exclusion Columns, 6-600 kDa have performance similar to Superdex™ 200 pg columns. G-Sep™ Size Exclusion Columns comes with adjustable end adaptors. G-Sep™ Dynamic Size Exclusion Columns comes in column format 16/60.

Highlights:

• High resolution preparative grade columns with separation range of 6-600 kDa for G-Sep™ Size Exclusion Column, 6-600 HR resin and 3-70 kDa for G-Sep™ Size Exclusion Column, 6-600 HR resin.
• The column is certified for Height Equivalent to a Theoretical Plate (HETP) and peak asymmetry (Theoretical Plate Number :>10,000 N/m and Peak Asymmetry: 0.7-1.5)

Features

• Matrix: Highly cross-linked polysaccharide composite of dextran and agarose.
• Mean Partice Size: 35 μm
• Column body maximum pressure: 6Bar, 0.6 MPa (3-70 HR columns); 5Bar, 0.5 MPa (6-600 HR columns)
• Maximun resin pressure: 4 Bar (3-70 HR columns); 3 Bar (6-600 HR columns)
• Operatin Flow velocity: 10-50 cm/hr
• pH stability: 2-14 (short term), 3-12 (long term)
• Working temperature: +4°C to +30°C 
• Chemical stability: Stable with commonly used aqueous buffers, 1 M cetic acid, 1 M NaoH, 6 M guanidine hydrochloride, 8 M urea. alcohol upto 30% v/v concentartion.
• Storage:20% ethanol, 4°C 

Application(s):

• G-Sep™ Size Exclusion Columns are used in sepration of biomolecules based on size (size exclusion chromatography)