menu
Your Cart

β-Galactosidase Assay (CPRG)

Catalog Description Size Price(USD) Qty
786-651
β-Galactosidase Assay (CPRG) Kit
$424.00

β-Galactosidase Assay (CPRG)

β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct.  The β-Galactosidase Assay (CPRG) assay uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic o-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays.  This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression.
 
β-galactosidase catalyzes the hydrolysis of the galactoside analog chlorophenol red-β-D-galactopyranoside (CPRG). Cell lysates are incubated with the supplied reagents and the β-Galactosidase converts the yellow-orange CPRG substrate into the red chromophore chlorophenol red, yielding a dark red solution. The β-galactosidase gene functions well as a reporter gene because the protein product is extremely stable, resistant to proteolytic degradation in cellular lysates, and easily assayed. The levels of activity β-galactosidase expression can be quickly measured by this method.
 
β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570–595 nm. Although the CPRG β-Galactosidase Assay Kit provides all the required reagents for 500 micro-assays in a 96-well plate, it is easily adapted for different assay size.
 
Features
  • Colorimetric Assay
  • ~10X More Sensitive than ONPG Assays
  • 500 Microwell Assays
  • Scalable

 

Applications

  • Monitor levels of transfection in cells
Protocol
786-651
Material Safety Data Sheet
786-651
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Write a review
Note: HTML is not translated!
Bad Good
Captcha

Download our handbooks

Custom Services

Connect With Us

Stay up to date with G-Biosciences by signing up for our newsletter.