ITA1091
ITA1091
ITA1091
from
$526.00
Price in reward points: 526
- Catalog: ITA1091
- Gene/Protein: MAP2K4
- Product Description: Immunotag™ Phospho-SEK1/MKK4 (Thr261) Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein Phospho-SEK1/MKK4 (Thr261) Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,IHC,ELISA Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 Concentration 1 mg/ml Reactive Species Human Host Species Rabbit Immunogen A synthesized peptide derived from human SEK1/MKK4 around the phosphorylation site of Threonine 261 Specificity Phospho-SEK1/MKK4 (Thr261) Antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at Threonine 261 Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns. Form Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt Gene Name MAP2K4 Accession No. P45985 Alternate Names c Jun N terminal kinase kinase 1; C-JUN N-terminal kinase kinase 1; Dual specificity mitogen activated protein kinase kinase 4; Dual specificity mitogen-activated protein kinase kinase 4; JNK Activated Kinase 1; JNK activating kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; Map2k4; MAPK ERK kinase 4; MAPK/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; Mitogen activated protein kinase kinase 4; MKK 4; MKK4; MP2K4_HUMAN; PRKMK4; SAPK ERK kinase 1; SAPK/ERK kinase 1; SAPKK 1; SAPKK1; SEK1; SERK1; SKK1; Stress activated protein kinase kinase 1; Description Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. Cell Pathway/ Category Primary Polyclonal Antibody Protein MW 44kDa Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet |
|
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | Phospho-SEK1/MKK4 (Thr261) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC,ELISA |
| Recommended Dilution | WB 1:500-1:2000 IHC 1:50-1:200 |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Rabbit |
| Immunogen | A synthesized peptide derived from human SEK1/MKK4 around the phosphorylation site of Threonine 261 |
| Specificity | Phospho-SEK1/MKK4 (Thr261) Antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at Threonine 261 |
| Purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns. |
| Form | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
| Gene Name | MAP2K4 |
| Accession No. | P45985 |
| Alternate Names | c Jun N terminal kinase kinase 1; C-JUN N-terminal kinase kinase 1; Dual specificity mitogen activated protein kinase kinase 4; Dual specificity mitogen-activated protein kinase kinase 4; JNK Activated Kinase 1; JNK activating kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; Map2k4; MAPK ERK kinase 4; MAPK/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; Mitogen activated protein kinase kinase 4; MKK 4; MKK4; MP2K4_HUMAN; PRKMK4; SAPK ERK kinase 1; SAPK/ERK kinase 1; SAPKK 1; SAPKK1; SEK1; SERK1; SKK1; Stress activated protein kinase kinase 1; |
| Description | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. |
| Cell Pathway/ Category | Primary Polyclonal Antibody |
| Protein MW | 44kDa |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |