ITA3804
ITA3804
- Catalog: ITA3804
- Gene/Protein: BRD4
- Product Description: Immunotag™ Brd4 Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet |
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| Target Protein | Brd4 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC,IF/ICC,ELISA |
| Recommended Dilution | WB 1:1000-3000 IHC 1:200 ICC/IF |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | A synthesized peptide derived from human Brd4 |
| Specificity | Brd4 Antibody detects endogenous levels of total Brd4 |
| Purification | The antiserum was purified by peptide affinity chromatography. |
| Form | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
| Gene Name | BRD4 |
| Accession No. | O60885 |
| Alternate Names | Brd4; BRD4-NUT FUSION; BRD4-NUT fusion oncoprotein; BRD4_HUMAN; Bromodomain containing 4; bromodomain containing protein 4; Bromodomain-containing protein 4; CAP; chromosome associated protein; HUNK1; HUNKI; MCAP; Mitotic chromosome-associated protein; Protein HUNK1; |
| Description | Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters. |
| Cell Pathway/ Category | Primary Polyclonal Antibody |
| Protein MW | 152kDa |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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