ITA3815
ITA3815
- Catalog: ITA3815
- Gene/Protein: DGCR8
- Product Description: Immunotag™ DGCR8 Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet |
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| Target Protein | DGCR8 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF/ICC,ELISA |
| Recommended Dilution | WB 1:1000-3000 ICC/IF |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | A synthesized peptide derived from human DGCR8 |
| Specificity | DGCR8 Antibody detects endogenous levels of total DGCR8 |
| Purification | The antiserum was purified by peptide affinity chromatography. |
| Form | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
| Gene Name | DGCR8 |
| Accession No. | Q8WYQ5 |
| Alternate Names | DGCRK6; C22orf12; D16H22S788E; D16Wis2; DGCR 8; Dgcr8; DGCR8 microprocessor complex subunit; DGCR8_HUMAN; DGCRK 6; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8; pasha; |
| Description | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity). |
| Cell Pathway/ Category | Primary Polyclonal Antibody |
| Protein MW | 100kDa |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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