ITA3815
ITA3815
ITA3815
from
$526.00
Price in reward points: 526
- Catalog: ITA3815
- Gene/Protein: DGCR8
- Product Description: Immunotag™ DGCR8 Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein DGCR8 Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,IF/ICC,ELISA Recommended Dilution WB 1:1000-3000 ICC/IF Concentration 1 mg/ml Reactive Species Human,Mouse,Rat Host Species Rabbit Immunogen A synthesized peptide derived from human DGCR8 Specificity DGCR8 Antibody detects endogenous levels of total DGCR8 Purification The antiserum was purified by peptide affinity chromatography. Form Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt Gene Name DGCR8 Accession No. Q8WYQ5 Alternate Names DGCRK6; C22orf12; D16H22S788E; D16Wis2; DGCR 8; Dgcr8; DGCR8 microprocessor complex subunit; DGCR8_HUMAN; DGCRK 6; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8; pasha; Description Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity). Cell Pathway/ Category Primary Polyclonal Antibody Protein MW 100kDa Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet |
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| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | DGCR8 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF/ICC,ELISA |
| Recommended Dilution | WB 1:1000-3000 ICC/IF |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | A synthesized peptide derived from human DGCR8 |
| Specificity | DGCR8 Antibody detects endogenous levels of total DGCR8 |
| Purification | The antiserum was purified by peptide affinity chromatography. |
| Form | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
| Gene Name | DGCR8 |
| Accession No. | Q8WYQ5 |
| Alternate Names | DGCRK6; C22orf12; D16H22S788E; D16Wis2; DGCR 8; Dgcr8; DGCR8 microprocessor complex subunit; DGCR8_HUMAN; DGCRK 6; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8; pasha; |
| Description | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity). |
| Cell Pathway/ Category | Primary Polyclonal Antibody |
| Protein MW | 100kDa |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |