ITA5619
ITA5619
- Catalog: ITA5619
- Gene/Protein: TOP1
- Product Description: Immunotag™ TOP1 Antibody
385.0000
Price in reward points: 385
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Antibody Specification | |
Datasheet | |
Target Protein | TOP1 |
Clonality | Polyclonal |
Storage/Stability | -20°C/1 year |
Application | WB,IF/ICC,ELISA |
Recommended Dilution | WB 1:500~1:1000, IF/ICC 1:100-1:500 |
Concentration | 1 mg/ml |
Reactive Species | Human,Mouse,Rat |
Host Species | Rabbit |
Immunogen | A synthesized peptide |
Specificity | TOP1 Antibody detects endogenous levels of total TOP1 |
Purification | The antiserum was purified by peptide affinity chromatography. |
Form | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
Gene Name | TOP1 |
Accession No. | P11387 |
Alternate Names | DNA topoisomerase 1; DNA topoisomerase I; NUP98 fusion gene; TOP 1; TOP I; TOP1; TOP1_HUMAN; TOPI; Topoisomerase (DNA) I; Topoisomerase 1; Topoisomerase1; TopoisomeraseI; Type I DNA topoisomerase; |
Description | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter. |
Cell Pathway/ Category | Primary Polyclonal Antibody |
Protein MW | 90 KD |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |