ITA7005
ITA7005
- Catalog: ITA7005
- Gene/Protein: DNMT3L
- Product Description: Immunotag™ DNMT3L Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet |
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| Target Protein | DNMT3L |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC,IF/ICC,IP,ELISA,CHIP |
| Recommended Dilution | WB 1:500-1:2000 IHC 1:50-1:200 IF 1:20-1:50 IP 1:20-1:50 CHIP 1:20-1:50 |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Rabbit |
| Immunogen | A synthesized peptide derived from human DNMT3L |
| Specificity | DNMT3L Antibody detects endogenous levels of total DNMT3L |
| Purification | The antiserum was purified by peptide affinity chromatography. |
| Form | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
| Gene Name | DNMT3L |
| Accession No. | Q9UJW3 |
| Alternate Names | Cytosine 5 methyltransferase 3 like protein; DNA (cytosine 5 ) methyltransferase 3 like; DNA (cytosine-5)-methyltransferase 3-like; DNA cytosine 5 methyltransferase 3 like protein; DNA methyltransferase 3 like protein; DNM3L_HUMAN; Dnmt 3L; Dnmt3l; Human cytosine 5 methyltransferase 3 like protein; MGC1090; |
| Description | Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327). Plays a key role in embryonic stem cells and germ cells (By similarity). In germ cells, required for the methylation of imprinted loci together with DNMT3A (By similarity). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (By similarity). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (By similarity). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (By similarity). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (By similarity). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity). |
| Cell Pathway/ Category | Primary Polyclonal Antibody |
| Protein MW | 44kDa |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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