ITM0070
ITM0070
ITM0070
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$556.00
Price in reward points: 556
- Catalog: ITM0070
- Gene/Protein: MCM2
- Product Description: Immunotag™ BM28 Monoclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein BM28 Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,IHC-p,IF,FCM,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human Host Species Mouse Immunogen Purified recombinant fragment of human BM28 expressed in E. Coli. Specificity BM28 Monoclonal Antibody detects endogenous levels of BM28 protein. Purification Affinity purification Form Ascitic fluid containing 0.03% sodium azide. Gene Name MCM2 Accession No. P49736 P97310 Alternate Names MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28 Description minichromosome maintenance complex component 2(MCM2) Homo sapiens The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012], Cell Pathway/ Category DNA replication,Cell_Cycle_G1S,Cell_Cycle_G2M_DNA, Protein Expression Bone marrow,Brain,Cervix carcinoma,Colon carcinoma,Epitheli Subcellular Localization nuclear chromosome, telomeric region,chromatin,nucleus,nucleoplasm,nuclear origin of replication recognition complex,cytoplasm,microtubule cytoskeleton,MCM complex, Protein Function function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.,PTM:Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,subunit:Interacts with DBF4 (By similarity). Interacts with MYST2. May interact with MCM10., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | BM28 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF,FCM,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human BM28 expressed in E. Coli. |
| Specificity | BM28 Monoclonal Antibody detects endogenous levels of BM28 protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | MCM2 |
| Accession No. | P49736 P97310 |
| Alternate Names | MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28 |
| Description | minichromosome maintenance complex component 2(MCM2) Homo sapiens The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012], |
| Cell Pathway/ Category | DNA replication,Cell_Cycle_G1S,Cell_Cycle_G2M_DNA, |
| Protein Expression | Bone marrow,Brain,Cervix carcinoma,Colon carcinoma,Epitheli |
| Subcellular Localization | nuclear chromosome, telomeric region,chromatin,nucleus,nucleoplasm,nuclear origin of replication recognition complex,cytoplasm,microtubule cytoskeleton,MCM complex, |
| Protein Function | function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.,PTM:Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,subunit:Interacts with DBF4 (By similarity). Interacts with MYST2. May interact with MCM10., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |