ITM0081
ITM0081
ITM0081
from
$556.00
Price in reward points: 556
- Catalog: ITM0081
- Gene/Protein: PTK6
- Product Description: Immunotag™ Brk Monoclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein BRK Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human Host Species Mouse Immunogen Purified recombinant fragment of human Brk expressed in E. Coli. Specificity Brk Monoclonal Antibody detects endogenous levels of Brk protein. Purification Affinity purification Form Ascitic fluid containing 0.03% sodium azide. Gene Name PTK6 Accession No. Q13882 Q64434 Alternate Names PTK6; BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK Description protein tyrosine kinase 6(PTK6) Homo sapiens The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012], Protein Expression Blood,Epithelium,Mammary tumor,Melanocyte,Urinary bladder, Subcellular Localization ruffle,nucleus,nucleoplasm,cytoplasm,cytosol,extrinsic component of cytoplasmic side of plasma membrane, Protein Function catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,enzyme regulation:Activated enzyme seems to have greater access to its substrates.,function:Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.,PTM:Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.,subunit:Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1. Interacts with phosphorylated IRS4.,tissue specificity:Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | BRK |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human Brk expressed in E. Coli. |
| Specificity | Brk Monoclonal Antibody detects endogenous levels of Brk protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | PTK6 |
| Accession No. | Q13882 Q64434 |
| Alternate Names | PTK6; BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK |
| Description | protein tyrosine kinase 6(PTK6) Homo sapiens The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012], |
| Protein Expression | Blood,Epithelium,Mammary tumor,Melanocyte,Urinary bladder, |
| Subcellular Localization | ruffle,nucleus,nucleoplasm,cytoplasm,cytosol,extrinsic component of cytoplasmic side of plasma membrane, |
| Protein Function | catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,enzyme regulation:Activated enzyme seems to have greater access to its substrates.,function:Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.,PTM:Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.,subunit:Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1. Interacts with phosphorylated IRS4.,tissue specificity:Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |