ITM0115
ITM0115
ITM0115
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- Catalog: ITM0115
- Gene/Protein: CD276
- Product Description: Immunotag™ CD276 Monoclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein CD276 Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,IHC-p,FCM,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human Host Species Mouse Immunogen Purified recombinant fragment of human CD276 expressed in E. Coli. Specificity CD276 Monoclonal Antibody detects endogenous levels of CD276 protein. Purification Affinity purification Form Ascitic fluid containing 0.03% sodium azide. Gene Name CD276 Accession No. Q5ZPR3 Q8VE98 Alternate Names CD276; B7H3; PSEC0249; CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD antigen CD276 Description CD276 molecule(CD276) Homo sapiens The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011], Cell Pathway/ Category Cell adhesion molecules (CAMs), Protein Expression Liver,Lung, Subcellular Localization external side of plasma membrane,integral component of membrane,extracellular exosome, Protein Function function:May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.,induction:By LPS in monocytes and by ionomycin in T and B lymphocytes. Up-regulated in cells mediating rejection of human transplants.,miscellaneous:B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.,similarity:Belongs to the immunoglobulin superfamily. BTN/MOG family.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,similarity:Contains 2 Ig-like V-type (immunoglobulin-like) domains.,subunit:Interacts with TREML2 and this interaction enhances T-cell activation.,tissue specificity:Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | CD276 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,FCM,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human CD276 expressed in E. Coli. |
| Specificity | CD276 Monoclonal Antibody detects endogenous levels of CD276 protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | CD276 |
| Accession No. | Q5ZPR3 Q8VE98 |
| Alternate Names | CD276; B7H3; PSEC0249; CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD antigen CD276 |
| Description | CD276 molecule(CD276) Homo sapiens The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011], |
| Cell Pathway/ Category | Cell adhesion molecules (CAMs), |
| Protein Expression | Liver,Lung, |
| Subcellular Localization | external side of plasma membrane,integral component of membrane,extracellular exosome, |
| Protein Function | function:May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.,induction:By LPS in monocytes and by ionomycin in T and B lymphocytes. Up-regulated in cells mediating rejection of human transplants.,miscellaneous:B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.,similarity:Belongs to the immunoglobulin superfamily. BTN/MOG family.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,similarity:Contains 2 Ig-like V-type (immunoglobulin-like) domains.,subunit:Interacts with TREML2 and this interaction enhances T-cell activation.,tissue specificity:Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |