ITM0173
ITM0173
ITM0173
from
$556.00
Price in reward points: 556
- Catalog: ITM0173
- Gene/Protein: CTCF
- Product Description: Immunotag™ CTCF Monoclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein CTCF Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Monkey Host Species Mouse Immunogen Purified recombinant fragment of human CTCF expressed in E. Coli. Specificity CTCF Monoclonal Antibody detects endogenous levels of CTCF protein. Purification Affinity purification Form Ascitic fluid containing 0.03% sodium azide. Gene Name CTCF Accession No. P49711 Q61164 Alternate Names CTCF; Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog Description CCCTC-binding factor(CTCF) Homo sapiens This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been Protein Expression Brain,Epithelium,Placenta, Subcellular Localization chromosome, centromeric region,condensed chromosome,nucleus,nucleoplasm,nucleolus, Protein Function function:Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.,similarity:Belongs to the CTCF zinc-finger protein family.,similarity:Contains 11 C2H2-type zinc fingers.,subunit:Interacts with CHD8.,tissue specificity:Ubiquitous. Absent in primary spermatocytes., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | CTCF |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Monkey |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human CTCF expressed in E. Coli. |
| Specificity | CTCF Monoclonal Antibody detects endogenous levels of CTCF protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | CTCF |
| Accession No. | P49711 Q61164 |
| Alternate Names | CTCF; Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog |
| Description | CCCTC-binding factor(CTCF) Homo sapiens This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been |
| Protein Expression | Brain,Epithelium,Placenta, |
| Subcellular Localization | chromosome, centromeric region,condensed chromosome,nucleus,nucleoplasm,nucleolus, |
| Protein Function | function:Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.,similarity:Belongs to the CTCF zinc-finger protein family.,similarity:Contains 11 C2H2-type zinc fingers.,subunit:Interacts with CHD8.,tissue specificity:Ubiquitous. Absent in primary spermatocytes., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |