ITM0649
ITM0649
ITM0649
from
$556.00
Price in reward points: 556
- Catalog: ITM0649
- Gene/Protein: WNT1
- Product Description: Immunotag™ Wnt-1 Monoclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein Wnt-1 Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,IHC-p,IF,FCM,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Mouse Host Species Mouse Immunogen Purified recombinant fragment of Wnt-1 expressed in E. Coli. Specificity Wnt-1 Monoclonal Antibody detects endogenous levels of Wnt-1 protein. Purification Affinity purification Form Ascitic fluid containing 0.03% sodium azide. Gene Name WNT1 Accession No. P04628 P04426 Alternate Names WNT1; INT1; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog Description Wnt family member 1(WNT1) Homo sapiens The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in Cell Pathway/ Category WNT,WNT-T CELLHedgehog,Melanogenesis,Pathways in cancer,Basal cell carcinoma, Protein Expression Testis, Subcellular Localization extracellular region,proteinaceous extracellular matrix,extracellular space,cytoplasm,endoplasmic reticulum lumen,Golgi lumen,plasma membrane,cell surface,endocytic vesicle membrane,extracellular exosome, Protein Function function:Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.,similarity:Belongs to the Wnt family.,subunit:Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | Wnt-1 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF,FCM,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of Wnt-1 expressed in E. Coli. |
| Specificity | Wnt-1 Monoclonal Antibody detects endogenous levels of Wnt-1 protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | WNT1 |
| Accession No. | P04628 P04426 |
| Alternate Names | WNT1; INT1; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog |
| Description | Wnt family member 1(WNT1) Homo sapiens The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in |
| Cell Pathway/ Category | WNT,WNT-T CELLHedgehog,Melanogenesis,Pathways in cancer,Basal cell carcinoma, |
| Protein Expression | Testis, |
| Subcellular Localization | extracellular region,proteinaceous extracellular matrix,extracellular space,cytoplasm,endoplasmic reticulum lumen,Golgi lumen,plasma membrane,cell surface,endocytic vesicle membrane,extracellular exosome, |
| Protein Function | function:Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.,similarity:Belongs to the Wnt family.,subunit:Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |