ITM1013
ITM1013
- Catalog: ITM1013
- Gene/Protein: ACLY
- Product Description: Immunotag™ ATP-citrate synthase Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | ATP-CS |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF,FCM |
| Recommended Dilution | Western Blot: 1/1000 - 1/2000. Immunofluorescence: 1/100 - 1/500. Flow cytometry: 1/100 - 1/200. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Bovine,Chicken,Pig,sheep |
| Host Species | Mouse |
| Immunogen | Purified recombinant human ATP-citrate synthase (C-terminus) protein fragments expressed in Ecoli |
| Specificity | ATP-citrate synthase Monoclonal Antibody detects endogenous levels of ATP-citrate synthase protein. |
| Purification | Affinity purification |
| Form | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol. |
| Gene Name | ACLY |
| Accession No. | P53396 Q91V92 P16638 |
| Alternate Names | ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme |
| Description | ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014], |
| Cell Pathway/ Category | Citrate cycle (TCA cycle), |
| Protein Expression | Brain,Epithelium,Hippocampus,Liver,Lymph,Platelet, |
| Subcellular Localization | nucleoplasm,cytoplasm,cytosol,plasma membrane,citrate lyase complex,membrane,extracellular exosome, |
| Protein Function | catalytic activity:ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.,function:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.,similarity:In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.,similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.,subunit:Homotetramer., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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