ITM1050
ITM1050
ITM1050
from
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Price in reward points: 556
- Catalog: ITM1050
- Gene/Protein: HK2
- Product Description: Immunotag™ HXK II Monoclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein HXK II Clonality Monoclonal Storage/Stability -20°C/1 year Application WB Recommended Dilution Western Blot: 1/1000 - 1/2000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Mouse,Rat,Pig Host Species Mouse Immunogen Purified recombinant human HXK II (N-terminus) protein fragments expressed in Ecoli Specificity HXK II Monoclonal Antibody detects endogenous levels of HXK II protein. Purification Affinity purification Form Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol. Gene Name HK2 Accession No. P52789 O08528 P27881 Alternate Names HK2; Hexokinase-2; Hexokinase type II; HK II; Muscle form hexokinase Description hexokinase 2(HK2) Homo sapiens Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009], Cell Pathway/ Category Glycolysis / Gluconeogenesis,Fructose and mannose metabolism,Galactose metabolism,Starch and sucrose metabolism,Amino sugar and nucleotide sugar metabolism,Insulin_Receptor,Type II diabetes mellitus, Protein Expression Blood,Endometrium carcinoma cell line,Muscle,Placenta,Skeletal muscle,Testis, Subcellular Localization cell,mitochondrial outer membrane,cytosol,membrane, Protein Function catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,polymorphism:Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-terminal sequence may be involved in membrane binding.,subunit:Monomer.,tissue specificity:Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | HXK II |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB |
| Recommended Dilution | Western Blot: 1/1000 - 1/2000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Pig |
| Host Species | Mouse |
| Immunogen | Purified recombinant human HXK II (N-terminus) protein fragments expressed in Ecoli |
| Specificity | HXK II Monoclonal Antibody detects endogenous levels of HXK II protein. |
| Purification | Affinity purification |
| Form | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol. |
| Gene Name | HK2 |
| Accession No. | P52789 O08528 P27881 |
| Alternate Names | HK2; Hexokinase-2; Hexokinase type II; HK II; Muscle form hexokinase |
| Description | hexokinase 2(HK2) Homo sapiens Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009], |
| Cell Pathway/ Category | Glycolysis / Gluconeogenesis,Fructose and mannose metabolism,Galactose metabolism,Starch and sucrose metabolism,Amino sugar and nucleotide sugar metabolism,Insulin_Receptor,Type II diabetes mellitus, |
| Protein Expression | Blood,Endometrium carcinoma cell line,Muscle,Placenta,Skeletal muscle,Testis, |
| Subcellular Localization | cell,mitochondrial outer membrane,cytosol,membrane, |
| Protein Function | catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,polymorphism:Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-terminal sequence may be involved in membrane binding.,subunit:Monomer.,tissue specificity:Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |