ITM1292
ITM1292
ITM1292
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$447.00
Price in reward points: 447
- Catalog: ITM1292
- Gene/Protein: cdk1
- Product Description: Immunotag™ CDC2/CDK1 mouse mAb
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein CDC2/CDK1 Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,ICC Recommended Dilution wb 1:100 icc 1:50 Concentration 1 mg/ml Reactive Species Human Host Species Mouse Immunogen Purified recombinant human CDC2/CDK1 protein fragments expressed in E.coli. Specificity This antibody detects endogenous levels of CDC2/CDK1 and does not cross-react with related proteins. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name cdk1 Accession No. P06493 P11440 Alternate Names Cdc 2;Cdc2;CDC28A;CDK 1;CDK1;CDK1_HUMAN;CDKN1;CELL CYCLE CONTROLLER CDC2;Cell division control protein 2;Cell division control protein 2 homolog;Cell division cycle 2 G1 to S and G2 to M;Cell division protein kinase 1;Cell Divsion Cycle 2 Protein;Cyclin Dependent Kinase 1;Cyclin-dependent kinase 1;DKFZp686L20222;MGC111195;p34 Cdk1;p34 protein kinase;P34CDC2. Description cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009], Cell Pathway/ Category Cell_Cycle_G1S,Cell_Cycle_G2M_DNA,Oocyte meiosis,p53,Gap junction,Progesterone-mediated oocyte maturation, Protein Expression Epithelium,Hippocampus,Mammary cancer,Skin,Small intestine, Subcellular Localization nuclear chromosome, telomeric region,nucleus,nucleoplasm,cytoplasm,mitochondrion,centrosome,cytosol,spindle microtubule,membrane,midbody,extracellular exosome,mitotic spindle, Protein Function catalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.,function:Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLGAP5. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | CDC2/CDK1 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ICC |
| Recommended Dilution | wb 1:100 icc 1:50 |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant human CDC2/CDK1 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of CDC2/CDK1 and does not cross-react with related proteins. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | cdk1 |
| Accession No. | P06493 P11440 |
| Alternate Names | Cdc 2;Cdc2;CDC28A;CDK 1;CDK1;CDK1_HUMAN;CDKN1;CELL CYCLE CONTROLLER CDC2;Cell division control protein 2;Cell division control protein 2 homolog;Cell division cycle 2 G1 to S and G2 to M;Cell division protein kinase 1;Cell Divsion Cycle 2 Protein;Cyclin Dependent Kinase 1;Cyclin-dependent kinase 1;DKFZp686L20222;MGC111195;p34 Cdk1;p34 protein kinase;P34CDC2. |
| Description | cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009], |
| Cell Pathway/ Category | Cell_Cycle_G1S,Cell_Cycle_G2M_DNA,Oocyte meiosis,p53,Gap junction,Progesterone-mediated oocyte maturation, |
| Protein Expression | Epithelium,Hippocampus,Mammary cancer,Skin,Small intestine, |
| Subcellular Localization | nuclear chromosome, telomeric region,nucleus,nucleoplasm,cytoplasm,mitochondrion,centrosome,cytosol,spindle microtubule,membrane,midbody,extracellular exosome,mitotic spindle, |
| Protein Function | catalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.,function:Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLGAP5. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |