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ITM1367

ITM1367
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ITM1367
ITM1367
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  • Catalog: ITM1367
  • Gene/Protein: ddb1
  • Product Description: Immunotag™ DDB1 mouse mAb

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  • Description
    Immunotag™ DDB1 mouse mAb
  • Specifications
    Antibody Specification
    Datasheet
    IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return.
    Target Protein DDB1
    Clonality Monoclonal
    Storage/Stability -20°C/1 year
    Application WB
    Recommended Dilution wb 1:1000
    Concentration 1 mg/ml
    Reactive Species Human,Mouse,Rat,Monkey
    Host Species Mouse
    Immunogen Purified recombinant human DDB1 protein fragments expressed in E.coli.
    Specificity This antibody detects endogenous levels of DDB1 and does not cross-react with related proteins.
    Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
    Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    Gene Name ddb1
    Accession No. Q16531 Q3U1J4
    Alternate Names Damage specific DNA binding protein 1;Damage-specific DNA-binding protein 1;DDB 1;DDB p127 subunit;Ddb1;DDB1_HUMAN;DDBa ;DNA damage binding protein 1;DNA damage-binding protein 1;DNA damage-binding protein a;HBV X-associated protein 1;UV damaged DNA binding factor;UV damaged DNA binding protein 1;UV DDB 1;UV DDB1;UV-damaged DNA-binding factor;UV-damaged DNA-binding protein 1;UV-DDB 1;UV-DDB1;X associated protein 1;XAP 1;XAP-1;XAP1;Xeroderma pigmentosum group E complementing protein;Xeroderma pigmentosum group E-complementing protein;XPCE;XPE;XPE BF;XPE binding factor;XPE-BF;XPE-binding factor.
    Description damage specific DNA binding protein 1(DDB1) Homo sapiens The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecul
    Cell Pathway/ Category Nucleotide excision repair,Ubiquitin mediated proteolysis,
    Protein Expression Brain,Epidermis,Fetal lung,Peripheral blood,Placenta,Skin,
    Subcellular Localization nuclear chromosome, telomeric region,extracellular space,nucleus,nucleoplasm,cytoplasm,Cul4A-RING E3 ubiquitin ligase complex,Cul4B-RING E3 ubiquitin ligase complex,extracellular exosome,Cul4-RING E3 ubiquitin ligase complex,
    Protein Function function:Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.,pathway:Protein modification; protein ubiquitination.,PTM:Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.,similarity:Belongs to the DDB1 family.,subcellular location:Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.,subunit:Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of numerous DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which consist of a core of DDB1, CUL4A or CUL4B and RBX1. DDB1 may recruit specific substrate targeting subunits to the DCX complex. These substrate targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Interacts with AMBRA1, ATG16L1, BTRC, C2ORF37, C4ORF30, DCAF15, DDA1, DET1, DTL, ERCC8, FBXW5, FBXW8, GRWD1, IQWD1, KATNB1, NLE1, NUP43, PAFAH1B1, PHIP, PWP1, RBBP4, RBBP5, RBBP7, RFWD2, SNRNP40, VPRBP, WDR5, WDR5B, WDR12, WDR21A, WDR22, WDR23, WDR26, WDR32, WDR39, WDR40A, WDR42, WDR42A, WDR53, WDR59, WDR61, WDR68, WSB1, WSB2 and WDTC1. DCX complexes may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. Interacts with NF2, TSC1 and TSC2. Interacts with Simian virus 5 protein V and the HBV X protein. Interaction with SV5 protein V may prevent the recruitment of DCAF proteins to DCX complexes.,
    Usage For Research Use Only! Not for diagnostic or therapeutic procedures.
  • Reviews
Immunotag™ DDB1 mouse mAb
Antibody Specification
Datasheet
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return.
Target Protein DDB1
Clonality Monoclonal
Storage/Stability -20°C/1 year
Application WB
Recommended Dilution wb 1:1000
Concentration 1 mg/ml
Reactive Species Human,Mouse,Rat,Monkey
Host Species Mouse
Immunogen Purified recombinant human DDB1 protein fragments expressed in E.coli.
Specificity This antibody detects endogenous levels of DDB1 and does not cross-react with related proteins.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene Name ddb1
Accession No. Q16531 Q3U1J4
Alternate Names Damage specific DNA binding protein 1;Damage-specific DNA-binding protein 1;DDB 1;DDB p127 subunit;Ddb1;DDB1_HUMAN;DDBa ;DNA damage binding protein 1;DNA damage-binding protein 1;DNA damage-binding protein a;HBV X-associated protein 1;UV damaged DNA binding factor;UV damaged DNA binding protein 1;UV DDB 1;UV DDB1;UV-damaged DNA-binding factor;UV-damaged DNA-binding protein 1;UV-DDB 1;UV-DDB1;X associated protein 1;XAP 1;XAP-1;XAP1;Xeroderma pigmentosum group E complementing protein;Xeroderma pigmentosum group E-complementing protein;XPCE;XPE;XPE BF;XPE binding factor;XPE-BF;XPE-binding factor.
Description damage specific DNA binding protein 1(DDB1) Homo sapiens The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecul
Cell Pathway/ Category Nucleotide excision repair,Ubiquitin mediated proteolysis,
Protein Expression Brain,Epidermis,Fetal lung,Peripheral blood,Placenta,Skin,
Subcellular Localization nuclear chromosome, telomeric region,extracellular space,nucleus,nucleoplasm,cytoplasm,Cul4A-RING E3 ubiquitin ligase complex,Cul4B-RING E3 ubiquitin ligase complex,extracellular exosome,Cul4-RING E3 ubiquitin ligase complex,
Protein Function function:Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.,pathway:Protein modification; protein ubiquitination.,PTM:Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.,similarity:Belongs to the DDB1 family.,subcellular location:Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.,subunit:Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of numerous DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which consist of a core of DDB1, CUL4A or CUL4B and RBX1. DDB1 may recruit specific substrate targeting subunits to the DCX complex. These substrate targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Interacts with AMBRA1, ATG16L1, BTRC, C2ORF37, C4ORF30, DCAF15, DDA1, DET1, DTL, ERCC8, FBXW5, FBXW8, GRWD1, IQWD1, KATNB1, NLE1, NUP43, PAFAH1B1, PHIP, PWP1, RBBP4, RBBP5, RBBP7, RFWD2, SNRNP40, VPRBP, WDR5, WDR5B, WDR12, WDR21A, WDR22, WDR23, WDR26, WDR32, WDR39, WDR40A, WDR42, WDR42A, WDR53, WDR59, WDR61, WDR68, WSB1, WSB2 and WDTC1. DCX complexes may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. Interacts with NF2, TSC1 and TSC2. Interacts with Simian virus 5 protein V and the HBV X protein. Interaction with SV5 protein V may prevent the recruitment of DCAF proteins to DCX complexes.,
Usage For Research Use Only! Not for diagnostic or therapeutic procedures.

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