ITM1388
ITM1388
- Catalog: ITM1388
- Gene/Protein: rpa2
- Product Description: Immunotag™ RPA32/RPA2 mouse mAb
405.0000
Price in reward points: 405
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | RPA32/RPA2 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IP,ICC |
| Recommended Dilution | wb dilution 1:2000 icc dilution 1:200 |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of RPA32/RPA2 and does not cross-react with related proteins. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | rpa2 |
| Accession No. | P15927 Q62193 |
| Alternate Names | 60S acidic ribosomal protein P1;AA409079;AI325195;AU020965;HSSB;ik:tdsubc_2g1;M(2)21C; MGC137236;OTTHUMP00000004008;p32;p34;RCJMB04_6d17 replication protein A2, 32kDa;REPA 2; REPA1;REPA2;Replication factor A protein 2;Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit;Replication protein A 34 kDa subunit;Replication protein A;replication protein A1 (70kD);Replication Protein A2 (32kDa);Replication protein A2 32kD;Replication protein A2 32kDa;Replication protein A2;Replication protein A2, 32kDa;RF A;RF-A protein 2;Rf-A2;RFA;RFA2_HUMAN;RP A;RP-A p32;RP-A p34;RP21C;RPA 2; RPA 32;RPA;RPA2;RPA32;RPA34;RPA70;RpLP1;RpP2;xx:tdsubc_2g1;zgc:109822. |
| Description | function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN., |
| Cell Pathway/ Category | DNA replication,Nucleotide excision repair,Mismatch repair,Homologous recombination, |
| Protein Expression | Kidney,Lung,Muscle, |
| Subcellular Localization | chromosome, telomeric region,nuclear chromosome, telomeric region,chromatin,nucleus,nucleoplasm,DNA replication factor A complex,PML body,site of double-strand break, |
| Protein Function | function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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