ITM1413
ITM1413
ITM1413
from
$447.00
Price in reward points: 447
- Catalog: ITM1413
- Gene/Protein: fen1
- Product Description: Immunotag™ FEN-1 mouse mAb
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein FEN-1 Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,ICC Recommended Dilution wb dilution 1:1000 icc dilution 1:400 Concentration 1 mg/ml Reactive Species Human,Mouse,Rat,Monkey,Hamster Host Species Mouse Immunogen Purified recombinant human FEN-1 protein fragments expressed in E.coli. Specificity This antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name fen1 Accession No. P39748 P39749 Alternate Names DNase IV;FEN-1;FEN1;FEN1_HUMAN;Flap endonuclease 1;Flap structure specific endonuclease 1;Flap structure-specific endonuclease 1;hFEN-1;hFEN1;Maturation factor 1;MF1;Rad2. Description flap structure-specific endonuclease 1(FEN1) Homo sapiens The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions Cell Pathway/ Category DNA replication,Base excision repair,Non-homologous end-joining, Protein Expression Breast,Leukemic T-cell,Lung, Subcellular Localization nuclear chromosome, telomeric region,nucleus,nucleoplasm,nucleolus,mitochondrion,plasma membrane,membrane,protein complex, Protein Function cofactor:Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.,function:Endonuclease that cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Also possesses 5' to 3' exonuclease activity on niked or gapped double-stranded DNA, and exhibits RNase H activity.,PTM:Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.,similarity:Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.,subunit:Interacts with PCNA. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | FEN-1 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ICC |
| Recommended Dilution | wb dilution 1:1000 icc dilution 1:400 |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Monkey,Hamster |
| Host Species | Mouse |
| Immunogen | Purified recombinant human FEN-1 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | fen1 |
| Accession No. | P39748 P39749 |
| Alternate Names | DNase IV;FEN-1;FEN1;FEN1_HUMAN;Flap endonuclease 1;Flap structure specific endonuclease 1;Flap structure-specific endonuclease 1;hFEN-1;hFEN1;Maturation factor 1;MF1;Rad2. |
| Description | flap structure-specific endonuclease 1(FEN1) Homo sapiens The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions |
| Cell Pathway/ Category | DNA replication,Base excision repair,Non-homologous end-joining, |
| Protein Expression | Breast,Leukemic T-cell,Lung, |
| Subcellular Localization | nuclear chromosome, telomeric region,nucleus,nucleoplasm,nucleolus,mitochondrion,plasma membrane,membrane,protein complex, |
| Protein Function | cofactor:Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.,function:Endonuclease that cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Also possesses 5' to 3' exonuclease activity on niked or gapped double-stranded DNA, and exhibits RNase H activity.,PTM:Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.,similarity:Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.,subunit:Interacts with PCNA. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |