ITM3127
- Catalog: ITM3127
- Gene/Protein: TAP-Tag
- Product Description: Immunotag™ TAP Tag Monoclonal Antibody(Mix)
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein Trx-Tag (5H9)
Clonality Monoclonal
Storage/Stability -20°C/1 year
Application WB
Recommended Dilution WB: 1:500-10000
Concentration 1 mg/ml
Reactive Species All
Host Species Mouse
Immunogen Recombinant Protein of TAP Tag
Specificity The antibody detects TAP recombinant protein.
Purification The antibody was affinity-purified from-Tag mouse ascyes by affinity-chromatography using specific immunogen.
Form PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Description The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. The TAP tag historically consists of a calmodulin binding peptide (CPB), a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP method including the combination of FLAG tags and HA tags. The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. The TAP tag has been especially useful and deployed with Yeast Tap-tagged ORF clones. These clones contain genomic fusions of the TAP construct and are extremely useful for determining natural protein interactions and expression level variations based on physiological changes.
Usage For Research Use Only! Not for diagnostic or therapeutic procedures.
- Reviews
| Antibody Specification | |
| Datasheet |
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| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | Trx-Tag (5H9) |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB |
| Recommended Dilution | WB: 1:500-10000 |
| Concentration | 1 mg/ml |
| Reactive Species | All |
| Host Species | Mouse |
| Immunogen | Recombinant Protein of TAP Tag |
| Specificity | The antibody detects TAP recombinant protein. |
| Purification | The antibody was affinity-purified from-Tag mouse ascyes by affinity-chromatography using specific immunogen. |
| Form | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Description | The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. The TAP tag historically consists of a calmodulin binding peptide (CPB), a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP method including the combination of FLAG tags and HA tags. The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. The TAP tag has been especially useful and deployed with Yeast Tap-tagged ORF clones. These clones contain genomic fusions of the TAP construct and are extremely useful for determining natural protein interactions and expression level variations based on physiological changes. |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |