ITM3162
ITM3162
ITM3162
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$507.00
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- Catalog: ITM3162
- Gene/Protein: HIST1H2BB
- Product Description: Immunotag™ Histone H2B Monoclonal Antibody(Mix)
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein Histone H2B (Mix) Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,IF, Recommended Dilution WB: 1:1000-3000 IF 1:200 Concentration 1 mg/ml Reactive Species Human,Mouse,Rat Host Species Mouse Immunogen Synthetic Peptide of Histone H2B Specificity The antibody detects endogenous Histone H2B protein. Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen Form PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Gene Name HIST1H2BB Accession No. P33778 Q64475 Alternate Names HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f Description histone cluster 1 H2B family member b(HIST1H2BB) Homo sapiens Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015], Cell Pathway/ Category Systemic lupus erythematosus, Protein Expression Epithelium, Subcellular Localization nuclear chromosome, telomeric region,nucleosome,nuclear nucleosome,nucleus,nucleoplasm,cytoplasm, Protein Function function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.,PTM:Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.,similarity:Belongs to the histone H2B family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA., Usage 0 - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | Histone H2B (Mix) |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF, |
| Recommended Dilution | WB: 1:1000-3000 IF 1:200 |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Mouse |
| Immunogen | Synthetic Peptide of Histone H2B |
| Specificity | The antibody detects endogenous Histone H2B protein. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Form | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Gene Name | HIST1H2BB |
| Accession No. | P33778 Q64475 |
| Alternate Names | HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f |
| Description | histone cluster 1 H2B family member b(HIST1H2BB) Homo sapiens Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015], |
| Cell Pathway/ Category | Systemic lupus erythematosus, |
| Protein Expression | Epithelium, |
| Subcellular Localization | nuclear chromosome, telomeric region,nucleosome,nuclear nucleosome,nucleus,nucleoplasm,cytoplasm, |
| Protein Function | function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.,PTM:Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.,similarity:Belongs to the histone H2B family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA., |
| Usage | 0 |