ITM3213
- Catalog: ITM3213
- Gene/Protein: eYFP-Tag
- Product Description: Immunotag™ EYFP-Tag Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein FLAG-Tag (2C5)
Clonality Polyclonal
Storage/Stability -20°C/1 year
Application WB
Recommended Dilution WB: 1:1000-3000
Concentration 1 mg/ml
Reactive Species All
Host Species Rabbit
Immunogen Recombinant Protein of EYFP-Tag
Specificity The antibody detects EYFP and YFP tag fusion proteins.
Purification The antibody was affinity-purified from mouse antiserum by affinity-chromatography using specific immunogen.
Form PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Description Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
Usage For Research Use Only! Not for diagnostic or therapeutic procedures.
- Reviews
| Antibody Specification | |
| Datasheet |
|
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | FLAG-Tag (2C5) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB |
| Recommended Dilution | WB: 1:1000-3000 |
| Concentration | 1 mg/ml |
| Reactive Species | All |
| Host Species | Rabbit |
| Immunogen | Recombinant Protein of EYFP-Tag |
| Specificity | The antibody detects EYFP and YFP tag fusion proteins. |
| Purification | The antibody was affinity-purified from mouse antiserum by affinity-chromatography using specific immunogen. |
| Form | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Description | Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore. |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |