ITM3462
ITM3462
ITM3462
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$424.00
Price in reward points: 424
- Catalog: ITM3462
- Gene/Protein: CCNB1
- Product Description: Immunotag™ Cyclin B1 Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein Cyclin B1 Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,IHC-p Recommended Dilution WB: 1:500-1:2000 IHC: 1:50-1:200 Concentration 1 mg/ml Reactive Species Human,Rat,Mouse Host Species Rabbit Immunogen Recombinant Protein of Cyclin B1 Specificity The antibody detects endogenous Cyclin B1 protein Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name CCNB1 Accession No. P14635 P24860 P30277 Alternate Names G2/mitotic-specific cyclin-B1 Description cyclin B1(CCNB1) Homo sapiens The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008], Cell Pathway/ Category Cell_Cycle_G1S,Cell_Cycle_G2M_DNA,Oocyte meiosis,p53,Progesterone-mediated oocyte maturation, Protein Expression Breast adenocarcinoma,Lung,Placenta, Subcellular Localization spindle pole,condensed nuclear chromosome outer kinetochore,nucleus,nucleoplasm,cytoplasm,centrosome,cytosol,membrane, Protein Function developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | Cyclin B1 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p |
| Recommended Dilution | WB: 1:500-1:2000 IHC: 1:50-1:200 |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Rat,Mouse |
| Host Species | Rabbit |
| Immunogen | Recombinant Protein of Cyclin B1 |
| Specificity | The antibody detects endogenous Cyclin B1 protein |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | CCNB1 |
| Accession No. | P14635 P24860 P30277 |
| Alternate Names | G2/mitotic-specific cyclin-B1 |
| Description | cyclin B1(CCNB1) Homo sapiens The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Cell_Cycle_G1S,Cell_Cycle_G2M_DNA,Oocyte meiosis,p53,Progesterone-mediated oocyte maturation, |
| Protein Expression | Breast adenocarcinoma,Lung,Placenta, |
| Subcellular Localization | spindle pole,condensed nuclear chromosome outer kinetochore,nucleus,nucleoplasm,cytoplasm,centrosome,cytosol,membrane, |
| Protein Function | developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |