ITN0594
ITN0594
ITN0594
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$424.00
Price in reward points: 424
- Catalog: ITN0594
- Gene/Protein: CHD8 HELSNF1 KIAA1564
- Product Description: Immunotag™ CHD8 Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein CHD8 Clonality Polyclonal Storage/Stability -20°C/1 year Application IHC-p Recommended Dilution IHC-p 1:50-300 Concentration 1 mg/ml Reactive Species Human Host Species Rabbit Immunogen Synthesized peptide derived from part region of human protein Specificity CHD8 Polyclonal Antibody detects endogenous levels of protein. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. Gene Name CHD8 HELSNF1 KIAA1564 Accession No. Q9HCK8 Q09XV5 Q9JIX5 Description chromodomain helicase DNA binding protein 8(CHD8) Homo sapiens This gene encodes a DNA helicase that functions as a transcription repressor by remodeling chromatin structure. It binds beta-catenin and negatively regulates Wnt signaling pathway, which plays a pivotal role in vertebrate early development and morphogenesis. Mice lacking this gene exhibit early embryonic death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010], Cell Pathway/ Category WNT,WNT-T CELL Protein Expression Brain,Duodenum,Epithelium,Liver,Lung,Lymph node,Spleen,Uter Subcellular Localization nucleus,nucleoplasm,protein complex,MLL1 complex, Protein Function function:DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.,miscellaneous:Its gene is located in the 14q11.2 region of the genome which is associated with developmental delay, cognitive impairment and similar minor anomalies in some children, suggesting that it may be a good candidate for the phenotype.,PTM:Sumoylated.,similarity:Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,subcellular location:Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.,subunit:Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3. Component of a multiprotein complex of 900 kDa containing WDR5., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | CHD8 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | IHC-p |
| Recommended Dilution | IHC-p 1:50-300 |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | CHD8 Polyclonal Antibody detects endogenous levels of protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Gene Name | CHD8 HELSNF1 KIAA1564 |
| Accession No. | Q9HCK8 Q09XV5 Q9JIX5 |
| Description | chromodomain helicase DNA binding protein 8(CHD8) Homo sapiens This gene encodes a DNA helicase that functions as a transcription repressor by remodeling chromatin structure. It binds beta-catenin and negatively regulates Wnt signaling pathway, which plays a pivotal role in vertebrate early development and morphogenesis. Mice lacking this gene exhibit early embryonic death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010], |
| Cell Pathway/ Category | WNT,WNT-T CELL |
| Protein Expression | Brain,Duodenum,Epithelium,Liver,Lung,Lymph node,Spleen,Uter |
| Subcellular Localization | nucleus,nucleoplasm,protein complex,MLL1 complex, |
| Protein Function | function:DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.,miscellaneous:Its gene is located in the 14q11.2 region of the genome which is associated with developmental delay, cognitive impairment and similar minor anomalies in some children, suggesting that it may be a good candidate for the phenotype.,PTM:Sumoylated.,similarity:Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,subcellular location:Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.,subunit:Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3. Component of a multiprotein complex of 900 kDa containing WDR5., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |