ITP0605
ITP0605
ITP0605
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$541.00
Price in reward points: 541
- Catalog: ITP0605
- Gene/Protein: BCAR1
- Product Description: Immunotag™ p130 Cas (phospho Tyr410) Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein p130 Cas (Tyr410) Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,IHC-p,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Mouse,Rat Host Species Rabbit Immunogen Synthesized phospho-peptide around the phosphorylation site of human p130 Cas (phospho Tyr410) Specificity Phospho-p130 Cas (Y410) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y410. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name BCAR1 Accession No. P56945 Q61140 Q63767 Alternate Names BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas Description BCAR1, Cas family scaffolding protein(BCAR1) Homo sapiens BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009], Cell Pathway/ Category Chemokine,Focal adhesion,Leukocyte transendothelial migration,Regulates Actin and Cytoskeleton, Protein Expression Caudate nucleus,Cerebellum,Cornea,Epithelium,Kidney,Lung,Mammary ca Subcellular Localization ruffle,nucleolus,cytoplasm,cytosol,plasma membrane,focal adhesion,actin cytoskeleton,lamellipodium, Protein Function domain:A serine-rich region promotes activation of the serum response element (SRE).,domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.,domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.,function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.,PTM:Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix.,similarity:Belongs to the CAS family.,similarity:Contains 1 SH3 domain.,subcellular location:Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.,subunit:Forms complexes in vivo with focal adhesion kinase 1, adapter protein CRKL and LYN kinase. Can heterodimerize with CASL. Interacts with BCAR3, NPHP1, PTK2B and SH2D3C (By similarity). Interacts with activated CSPG4. Interacts with INPPL1/SHIP2.,tissue specificity:Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | p130 Cas (Tyr410) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human p130 Cas (phospho Tyr410) |
| Specificity | Phospho-p130 Cas (Y410) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y410. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | BCAR1 |
| Accession No. | P56945 Q61140 Q63767 |
| Alternate Names | BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas |
| Description | BCAR1, Cas family scaffolding protein(BCAR1) Homo sapiens BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009], |
| Cell Pathway/ Category | Chemokine,Focal adhesion,Leukocyte transendothelial migration,Regulates Actin and Cytoskeleton, |
| Protein Expression | Caudate nucleus,Cerebellum,Cornea,Epithelium,Kidney,Lung,Mammary ca |
| Subcellular Localization | ruffle,nucleolus,cytoplasm,cytosol,plasma membrane,focal adhesion,actin cytoskeleton,lamellipodium, |
| Protein Function | domain:A serine-rich region promotes activation of the serum response element (SRE).,domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.,domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.,function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.,PTM:Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix.,similarity:Belongs to the CAS family.,similarity:Contains 1 SH3 domain.,subcellular location:Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.,subunit:Forms complexes in vivo with focal adhesion kinase 1, adapter protein CRKL and LYN kinase. Can heterodimerize with CASL. Interacts with BCAR3, NPHP1, PTK2B and SH2D3C (By similarity). Interacts with activated CSPG4. Interacts with INPPL1/SHIP2.,tissue specificity:Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |