ITP0623
ITP0623
ITP0623
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$541.00
Price in reward points: 541
- Catalog: ITP0623
- Gene/Protein: CDC6
- Product Description: Immunotag™ Cdc6 (phospho Ser54) Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein CDC6 (Ser54) Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,IHC-p,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Mouse,Monkey Host Species Rabbit Immunogen The antiserum was produced against synthesized peptide derived from human CDC6 around the phosphorylation site of Ser54. AA range:20-69 Specificity Phospho-Cdc6 (S54) Polyclonal Antibody detects endogenous levels of Cdc6 protein only when phosphorylated at S54. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name CDC6 Accession No. Q99741 O89033 Alternate Names CDC6; CDC18L; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6 Description cell division cycle 6(CDC6) Homo sapiens The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008], Cell Pathway/ Category Cell_Cycle_G1S,Cell_Cycle_G2M_DNA, Protein Expression Brain,Epithelium, Subcellular Localization spindle pole,nucleus,nucleoplasm,cytoplasm,Golgi apparatus,cytosol,spindle midzone, Protein Function function:Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.,similarity:Belongs to the CDC6/cdc18 family.,subcellular location:The protein is nuclear in G1 and cytoplasmic in S-phase cells.,subunit:Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | CDC6 (Ser54) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Monkey |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDC6 around the phosphorylation site of Ser54. AA range:20-69 |
| Specificity | Phospho-Cdc6 (S54) Polyclonal Antibody detects endogenous levels of Cdc6 protein only when phosphorylated at S54. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | CDC6 |
| Accession No. | Q99741 O89033 |
| Alternate Names | CDC6; CDC18L; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6 |
| Description | cell division cycle 6(CDC6) Homo sapiens The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Cell_Cycle_G1S,Cell_Cycle_G2M_DNA, |
| Protein Expression | Brain,Epithelium, |
| Subcellular Localization | spindle pole,nucleus,nucleoplasm,cytoplasm,Golgi apparatus,cytosol,spindle midzone, |
| Protein Function | function:Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.,similarity:Belongs to the CDC6/cdc18 family.,subcellular location:The protein is nuclear in G1 and cytoplasmic in S-phase cells.,subunit:Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |