ITP0661
ITP0661
ITP0661
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$541.00
Price in reward points: 541
- Catalog: ITP0661
- Gene/Protein: MYB
- Product Description: Immunotag™ c-Myb (phospho Ser12) Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein c-Myb (Ser12) Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,IHC-p,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Mouse Host Species Rabbit Immunogen The antiserum was produced against synthesized peptide derived from human MYB around the phosphorylation site of Ser12. AA range:1-50 Specificity Phospho-c-Myb (S12) Polyclonal Antibody detects endogenous levels of c-Myb protein only when phosphorylated at S12. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name MYB Accession No. P10242 P06876 Alternate Names MYB; Transcriptional activator Myb; Proto-oncogene c-Myb Description MYB proto-oncogene, transcription factor(MYB) Homo sapiens This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016], Cell Pathway/ Category PI3K/Akt, Protein_Acetylation Protein Expression Liver,Placenta,Testis, Subcellular Localization nucleus,integral component of membrane,nuclear matrix, Protein Function domain:Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.,function:Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.,PTM:Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.,PTM:Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.,similarity:Contains 3 HTH myb-type DNA-binding domains.,subunit:Binds MYBBP1A. Interacts with HIPK2, MAF and NLK., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | c-Myb (Ser12) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MYB around the phosphorylation site of Ser12. AA range:1-50 |
| Specificity | Phospho-c-Myb (S12) Polyclonal Antibody detects endogenous levels of c-Myb protein only when phosphorylated at S12. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | MYB |
| Accession No. | P10242 P06876 |
| Alternate Names | MYB; Transcriptional activator Myb; Proto-oncogene c-Myb |
| Description | MYB proto-oncogene, transcription factor(MYB) Homo sapiens This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016], |
| Cell Pathway/ Category | PI3K/Akt, Protein_Acetylation |
| Protein Expression | Liver,Placenta,Testis, |
| Subcellular Localization | nucleus,integral component of membrane,nuclear matrix, |
| Protein Function | domain:Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.,function:Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.,PTM:Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.,PTM:Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.,similarity:Contains 3 HTH myb-type DNA-binding domains.,subunit:Binds MYBBP1A. Interacts with HIPK2, MAF and NLK., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |