ITP0869
ITP0869
ITP0869
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$541.00
Price in reward points: 541
- Catalog: ITP0869
- Gene/Protein: CRKL
- Product Description: Immunotag™ Crk-L (phospho Tyr207) Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein Crk-L (Tyr207) Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,IHC-p,IF,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Mouse,Rat,Monkey Host Species Rabbit Immunogen Synthesized phospho-peptide around the phosphorylation site of human Crk-L (phospho Tyr207) Specificity Phospho-Crk-L (Y207) Polyclonal Antibody detects endogenous levels of Crk-L protein only when phosphorylated at Y207. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name CRKL Accession No. P46109 P47941 Q5U2U2 Alternate Names CRKL; Crk-like protein Description CRK like proto-oncogene, adaptor protein(CRKL) Homo sapiens This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009], Cell Pathway/ Category MAPK_ERK_Growth,MAPK_G_Protein,ErbB_HER,Chemokine,Focal adhesion,Fc gamma R-mediated phagocytosis,Neurotrophin,Regulates Actin and Cytoskeleton,Insulin_Receptor,Pathways in cancer,Renal cell carcinoma,Chronic myeloid leukemia, Protein Expression Skin,Spleen, Subcellular Localization endosome,cytosol,cell-cell adherens junction,extracellular exosome, Protein Function function:May mediate the transduction of intracellular signals.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | Crk-L (Tyr207) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Monkey |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Crk-L (phospho Tyr207) |
| Specificity | Phospho-Crk-L (Y207) Polyclonal Antibody detects endogenous levels of Crk-L protein only when phosphorylated at Y207. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | CRKL |
| Accession No. | P46109 P47941 Q5U2U2 |
| Alternate Names | CRKL; Crk-like protein |
| Description | CRK like proto-oncogene, adaptor protein(CRKL) Homo sapiens This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009], |
| Cell Pathway/ Category | MAPK_ERK_Growth,MAPK_G_Protein,ErbB_HER,Chemokine,Focal adhesion,Fc gamma R-mediated phagocytosis,Neurotrophin,Regulates Actin and Cytoskeleton,Insulin_Receptor,Pathways in cancer,Renal cell carcinoma,Chronic myeloid leukemia, |
| Protein Expression | Skin,Spleen, |
| Subcellular Localization | endosome,cytosol,cell-cell adherens junction,extracellular exosome, |
| Protein Function | function:May mediate the transduction of intracellular signals.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |