ITP0870
ITP0870
ITP0870
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- Catalog: ITP0870
- Gene/Protein: EEF2
- Product Description: Immunotag™ EF-2 (phospho Thr56) Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein EF-2 (Thr56) Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,IHC-p,IF,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Mouse,Rat Host Species Rabbit Immunogen Synthesized phospho-peptide around the phosphorylation site of human EF-2 (phospho Thr56) Specificity Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name EEF2 Accession No. P13639 P58252 P05197 Alternate Names EEF2; EF2; Elongation factor 2; EF-2 Description eukaryotic translation elongation factor 2(EEF2) Homo sapiens This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008], Cell Pathway/ Category AMPK Protein Expression Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph Subcellular Localization nucleus,cytoplasm,cytosol,plasma membrane,cell-cell adherens junction,membrane,aggresome,intracellular ribonucleoprotein complex,extracellular matrix,polysomal ribosome,membrane raft,extracellular exosome Protein Function function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | EF-2 (Thr56) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human EF-2 (phospho Thr56) |
| Specificity | Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | EEF2 |
| Accession No. | P13639 P58252 P05197 |
| Alternate Names | EEF2; EF2; Elongation factor 2; EF-2 |
| Description | eukaryotic translation elongation factor 2(EEF2) Homo sapiens This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | AMPK |
| Protein Expression | Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph |
| Subcellular Localization | nucleus,cytoplasm,cytosol,plasma membrane,cell-cell adherens junction,membrane,aggresome,intracellular ribonucleoprotein complex,extracellular matrix,polysomal ribosome,membrane raft,extracellular exosome |
| Protein Function | function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |