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ITP1131

ITP1131
  • Gene/Protein: PPP1R12A
  • Product Description: Immunotag™ MYPT1 (phospho Thr696) Polyclonal Antibody
357.0000
Price in reward points: 357

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Immunotag™ MYPT1 (phospho Thr696) Polyclonal Antibody
Antibody Specification
Datasheet
Target Protein MYPT1 (Thr696)
Clonality Polyclonal
Storage/Stability -20°C/1 year
Application IHC-p,IF,ELISA
Recommended Dilution Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Concentration 1 mg/ml
Reactive Species Human,Mouse,Rat
Host Species Rabbit
Immunogen Synthesized phospho-peptide around the phosphorylation site of human MYPT1 (phospho Thr696)
Specificity Phospho-MYPT1 (T696) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T696.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene Name PPP1R12A
Accession No. O14974 Q9DBR7 Q10728
Alternate Names PPP1R12A; MBS; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit
Description protein phosphatase 1 regulatory subunit 12A(PPP1R12A) Homo sapiens Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosph
Cell Pathway/ Category Vascular smooth muscle contraction,Focal adhesion,Long-term potentiation,Regulates Actin and Cytoskeleton,
Protein Expression Blood,Brain,Colon,Epithelium,Liver,Placenta,Platelet,T-cell
Subcellular Localization kinetochore,nucleoplasm,cytoplasm,centrosome,cytosol,focal adhesion,actin cytoskeleton,Z disc,A band,contractile fiber,PTW/PP1 phosphatase complex,
Protein Function function:Regulates myosin phosphatase activity.,PTM:Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 6 ANK repeats.,subcellular location:Along actomyosin filaments and stress fibers.,subunit:PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.,
Usage For Research Use Only! Not for diagnostic or therapeutic procedures.
Material Safety Data Sheet
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