ITT3088
ITT3088
ITT3088
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- Catalog: ITT3088
- Gene/Protein: NEFM
- Product Description: Immunotag™ NF-M Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein NFM Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human Host Species Rabbit Immunogen The antiserum was produced against synthesized peptide derived from human NF-M. AA range:542-591 Specificity NF-M Polyclonal Antibody detects endogenous levels of NF-M protein. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name NEFM Accession No. P07197 P08553 Alternate Names NEFM; NEF3; NFM; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein Description neurofilament, medium polypeptide(NEFM) Homo sapiens Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008], Cell Pathway/ Category Amyotrophic lateral sclerosis (ALS), Protein Expression Brain,Brain cortex,Fetal brain cortex, Subcellular Localization neurofilament,axon,neurofibrillary tangle, Protein Function function:Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.,PTM:Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.,PTM:There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.,similarity:Belongs to the intermediate filament family., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | NFM |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-M. AA range:542-591 |
| Specificity | NF-M Polyclonal Antibody detects endogenous levels of NF-M protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | NEFM |
| Accession No. | P07197 P08553 |
| Alternate Names | NEFM; NEF3; NFM; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein |
| Description | neurofilament, medium polypeptide(NEFM) Homo sapiens Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008], |
| Cell Pathway/ Category | Amyotrophic lateral sclerosis (ALS), |
| Protein Expression | Brain,Brain cortex,Fetal brain cortex, |
| Subcellular Localization | neurofilament,axon,neurofibrillary tangle, |
| Protein Function | function:Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.,PTM:Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.,PTM:There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.,similarity:Belongs to the intermediate filament family., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |