ITT4210
ITT4210
ITT4210
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- Catalog: ITT4210
- Gene/Protein: SF3B14
- Product Description: Immunotag™ SAP 14 Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein SAP 14 Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human,Mouse Host Species Rabbit Immunogen The antiserum was produced against synthesized peptide derived from human SF3B14. AA range:76-125 Specificity SAP 14 Polyclonal Antibody detects endogenous levels of SAP 14 protein. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name SF3B14 Accession No. Q9Y3B4 P59708 Alternate Names SF3B14; CGI-110; HSPC175; HT006; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit Description splicing factor 3b subunit 6(SF3B6) Homo sapiens This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008], Cell Pathway/ Category Spliceosome, Protein Expression Hypothalamus,Testis,Umbilical cord blood,Uterus, Subcellular Localization nucleoplasm,U2-type spliceosomal complex,U2 snRNP,U12-type spliceosomal complex,precatalytic spliceosome,catalytic step 2 spliceosome, Protein Function function:Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with SF3B1/SF3b155 in the region of amino acids 255-424. Interacts to a lesser extent with SF3b130., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | SAP 14 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SF3B14. AA range:76-125 |
| Specificity | SAP 14 Polyclonal Antibody detects endogenous levels of SAP 14 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | SF3B14 |
| Accession No. | Q9Y3B4 P59708 |
| Alternate Names | SF3B14; CGI-110; HSPC175; HT006; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit |
| Description | splicing factor 3b subunit 6(SF3B6) Homo sapiens This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Spliceosome, |
| Protein Expression | Hypothalamus,Testis,Umbilical cord blood,Uterus, |
| Subcellular Localization | nucleoplasm,U2-type spliceosomal complex,U2 snRNP,U12-type spliceosomal complex,precatalytic spliceosome,catalytic step 2 spliceosome, |
| Protein Function | function:Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with SF3B1/SF3b155 in the region of amino acids 255-424. Interacts to a lesser extent with SF3b130., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |