LB Broth [Miller] Dry

LB Broth, Miller formulation, is widely used microbial growth medium used for cultivation of E.coli. The medium is rich in nutrients such as peptides, amino acids, water-soluble vitamins and carbohydrates. The medium is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. In addition suitable for selective cultivation when appropriate antibiotics are added. LB Broth [Miller] Dry is the non-sterile powder of Miller LB Broth.

Click here to request bulk or custom sizes.

Features:

• Ready to use bacterial cell culture medium.
• Composition: 1% Tryptone, 0.5% Yeast Extract, 1%NaCl
• Available in 250 g and 4 x 250 g sizes.

Preparation note: 

LB Broth:  Weigh 25g of LB Broth [Miller] Dry and transfer it to bottle with stir bar. Add 900 ml deionized water. Stir to mix. Check and adjust the pH to 7±0.2 if required. Make up to final volume (1L). Autoclave at 121°C (15 psi) for 15 minutes.

Application(s):

• LB Broth, Miller formulation, is microbial growth medium used for cultivation of E.coli for various purposes such as cloning, recombinant protein production and DNA plasmid purification.