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InGel™ Array

Catalog Description Size Price(USD) Qty
786-241A
InGel™ Array 500 Preps
$476.00

InGel™ Array

A 96-well format kit suitable for processing larger numbers of protein spots concurrently and compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.

 



Excise protein spots and transfer to the supplied proteomic grade titer plate, destain silver stained gel pieces with SilverOUT™ and then incubate with our proprietary Trypsin-Digestion Buffer Mix, a mixture of MSG-Trypsin™ and an optimal digestion buffer, which ensures reliable and efficient protein digestion.

 



The resulting digested peptides are extracted with Pep-Extract™, a high diffusion peptide extraction buffer. The extracted peptides are suitable for mass spectrometry analysis without the need for further cleaning procedures.

 



Each kit is supplied with:

  • SilverOUT™ destaining reagent.
  • Trypsin Digestion Buffer for optimal trypsin activity.
  • Pep-Extract™ Buffer, for high level peptide extraction.
  • InGel™ Array titer plates and caps

 


The above components are also available individually for added convenience. See the Accessories Section. MSG-Trypsin™, a mass spectrometry grade trypsin with minimal autolysis and increases stability, is available separately, see below.

 


Features

  • Digestion of protein spots in 96 well titer plate format.
  • Supplied with reagents sufficient for up to 500 protein spots.
  • Suitable for 2D gels stained with silver stain, Coomassie dye, and other methods.

 


Applications

  • Digestion of protein spots for mass spectrometry applications.
  • Suitable for 96 well titer plate applications.
  • Suitable for high throughput applications.
Protocol
786-241A
Material Safety Data Sheet
786-241A
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
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