| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-11 | SG-Arginine-C™ | 2 x 10µg | $453.00 |
SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyzes the carboxy peptide bond of Arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilize enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
SG-Arginine-C™ is supplied lyophilized in an activated form in 10μg vials and can be reconstituted to a concentration of 5ng/μl. For fragmentation the enzyme is added to the sample protein in a ratio of 1:10 to 1:20 (enzyme to protein, by weight)
Features
Applications
SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyzes the carboxy peptide bond of Arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilize enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
SG-Arginine-C™ is supplied lyophilized in an activated form in 10μg vials and can be reconstituted to a concentration of 5ng/μl. For fragmentation the enzyme is added to the sample protein in a ratio of 1:10 to 1:20 (enzyme to protein, by weight)
Features
Applications
