Micro Bicinchoninic Acid (BCA) Protein Assay

Micro Bicinchoninic Acid (BCA) Protein Assay

The Micro Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0.5-20µg/ml).

 

The Micro BCA Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid chelate with each Cu+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562nm. The assay uses concentrated solutions and extended incubation times for the detection of dilute protein samples. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations.

 

The Micro BCA Protein Assay is suitable for quantifying protein solutions in 500 x 1ml assays or in >3,300 micro-well assays.

 

The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.

 

For information on our regular Bicinchoninic Acid (BCA) Protein Assay, click here.

 

For samples with reducing agents, we recommend the Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay. 

 


Features

  • Sensitive, colorimetric assay for dilute protein samples
  • Linear range: 0.5-20µg/ml
  • Compatible with most non-ionic and ionic detergents

 



Applications

  • For dilute protein samples (0.5-20µg/ml)

 

 

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Protocol
786-572
786-861
786-862
786-895
Material Safety Data Sheet
786-572
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786-861
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
786-862
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
786-895
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Technical Literature
A Non-Animal Alternative to BSA Protein Standards
Bioassays Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Catalog Description Size Price(USD) Qty
786-572
Micro Bicinchoninic Acid (BCA) Protein Assay 500 Assays/ 3,300 Micro-assays
$204.00
786-861
Assay Buffer (Micro BCA Reagent) 250ml
$52.00
786-862
Micro BCA Solution (BCA Reagent) 240ml
$94.00
786-895
Micro Bicinchoninic Acid (BCA) Protein Assay with Non Animal Protein Standard 500 Assays/ 3,300 Micro-assays
$204.00