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TBST [10X]

TBST [10X]
TBST [10X]
TBST [10X]
Catalog Description Size Price (USD) Qty
786-1742JAW™ TBS with Tween 2020 x 1L Packs$110.00
R042TBST [10X]; Tris buffered saline with Tween-20; 100mM Tris.HCl, 1L$72.00

 A 10X Concentrated solution of Tris Buffered Saline with Tween® 20 with a concentration of 100mM Tris.HCl, 1500mM NaCl, 0.5% Tween® 20 at pH7.5. The 1X Concentration is 10mM Tris.HCl, 150mM NaCl, 0.05% Tween® 20 at pH7.5. A dry buffer pack format is also avaialble to produce 1L of 1X TBST.

TBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Tris Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Features

  •  1X Concentration is 10mM Tris.HCl, 150mM NaCl, 0.05% Tween® 20 at pH7.5

Applications

  •  Wash buffer for Western Blotting and ELISA

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