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Agarose IV (Highest Gel Strength)

Agarose IV (Highest Gel Strength)
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Agarose IV (Highest Gel Strength)
Agarose IV (Highest Gel Strength)
Catalog Description Size Price (USD) Qty
RC-011Agarose IV ( Highest Gel Strength)25g$108.00
RC-012Agarose IV ( Highest Gel Strength)100g$356.00
RC-036Agarose IV ( Highest Gel Strength)500g$1,625.00
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  • Description
    Agarose IV is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses.  This characteristic produces a strong intercatenary interaction, yeilding a gel with very high gel strength and higher exclusion limit.
     
    For improved resolution, we recommend storing the gel at 4°C for 30 minutes before use.

     

    Specifications:

    • Moisture: ≤ 7%
    • Ash: ≤ 0.25%
    • EEO (Electroendosmosis): ≤ 0.12
    • Sulfate: ≤ 0.12%
    • Clarity (NTU): ≤ 4
    • Gel Strength 1% (g/cm²): ≥ 1800
    • Gel Strength 1.5% (g/cm²): ≥ 3200
    • Gelling Temperature 3% (°C): ≤ 36±1.5
    • Melting Temperature 3% (°C): ≤ 88±1.5
    • DNase/ RNase Activity: None Detected
    • Gel Background: Very Low 

     

    Appropriate Agarose Concentrations:

    Gel Concentration TAE Buffer [1X]

    1.8%

    		 200-5,000bp
    1% 400-10,000bp
    0.8% 1,000-15,000bp
    0.5% 2,000-20,000bp
    0.3% 3,000-40,000bp

    Migration in the TBE Buffer is slower, therefore lower concentrations can be used to obtain similar separation ranges.

     


    Applications:

    • Conventional Electrophoresis
    • Pulsed Field Gel Electrophoresis: Due to its higher exclusion limit, large molecules can be separated
    • Blotting
    • Agarose Bead Preparation
    • Cell and Enzyme Immobilization

     

     

  • Reviews
Agarose IV is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses.  This characteristic produces a strong intercatenary interaction, yeilding a gel with very high gel strength and higher exclusion limit.
 
For improved resolution, we recommend storing the gel at 4°C for 30 minutes before use.

 

Specifications:

  • Moisture: ≤ 7%
  • Ash: ≤ 0.25%
  • EEO (Electroendosmosis): ≤ 0.12
  • Sulfate: ≤ 0.12%
  • Clarity (NTU): ≤ 4
  • Gel Strength 1% (g/cm²): ≥ 1800
  • Gel Strength 1.5% (g/cm²): ≥ 3200
  • Gelling Temperature 3% (°C): ≤ 36±1.5
  • Melting Temperature 3% (°C): ≤ 88±1.5
  • DNase/ RNase Activity: None Detected
  • Gel Background: Very Low 

 

Appropriate Agarose Concentrations:

Gel Concentration TAE Buffer [1X]

1.8%

 200-5,000bp
1% 400-10,000bp
0.8% 1,000-15,000bp
0.5% 2,000-20,000bp
0.3% 3,000-40,000bp

Migration in the TBE Buffer is slower, therefore lower concentrations can be used to obtain similar separation ranges.

 


Applications:

  • Conventional Electrophoresis
  • Pulsed Field Gel Electrophoresis: Due to its higher exclusion limit, large molecules can be separated
  • Blotting
  • Agarose Bead Preparation
  • Cell and Enzyme Immobilization

 

 

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