| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-2007 | T4 Polynucleotide Kinase (PNK) | 500 U | $50.00 | |
| 786-2008 | T4 Polynucleotide Kinase (PNK) | 2500 U | $164.00 | |
| 786-2009 | T4 Polynucleotide Kinase (PNK) | 5000 U | $297.00 |
Polynucleotide Kinase (PNK) exhibits 5’ polynucleotide kinase and 3’ phosphatase activity. It catalyzes 5’-phosphorylation of DNA and oligonucleotides via transferring the terminal phosphate of ATP to a 5’ hydroxyl group of a nucleic acid.
Source
E. coli strain expressing a cloned pseT gene of bacteriophage T4.
Includes
10X PNK Reaction Buffer
500 mM Tris-HCl, 100 mM MgCl2, 50 mM dithiothreitol, pH 7.5 @ 25°C.
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC.
Unit Definition:
One unit of T4 Polynucleotide Kinase converts 1nmol of 32P from [γ-32P]-ATP into an acid-insoluble form in 30 minutes at 37°C under standard assay conditions.
Molecular Weight
Theoretical: 34.6 kDa
Storage temperature
-20°C.
Inactivation
Inactivates by heating at 70°C for 15 min.
Inhibition
Metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50 mM can inhibit PNK
Quality Control Assays
T4 PNK is free from detectable nuclease and RNase contamination.
Polynucleotide Kinase (PNK) exhibits 5’ polynucleotide kinase and 3’ phosphatase activity. It catalyzes 5’-phosphorylation of DNA and oligonucleotides via transferring the terminal phosphate of ATP to a 5’ hydroxyl group of a nucleic acid.
Source
E. coli strain expressing a cloned pseT gene of bacteriophage T4.
Includes
10X PNK Reaction Buffer
500 mM Tris-HCl, 100 mM MgCl2, 50 mM dithiothreitol, pH 7.5 @ 25°C.
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC.
Unit Definition:
One unit of T4 Polynucleotide Kinase converts 1nmol of 32P from [γ-32P]-ATP into an acid-insoluble form in 30 minutes at 37°C under standard assay conditions.
Molecular Weight
Theoretical: 34.6 kDa
Storage temperature
-20°C.
Inactivation
Inactivates by heating at 70°C for 15 min.
Inhibition
Metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50 mM can inhibit PNK
Quality Control Assays
T4 PNK is free from detectable nuclease and RNase contamination.
