| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-2195 | T5 Exonuclease | 1000 U | $75.00 | |
| 786-2196 | T5 Exonuclease | 5000 U | $295.00 |
T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA. The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5´→3´ exonuclease activity of E. coli DNA Polymerase I.
Product Source:
Product Includes:
Applications:
T5 Exonuclease reaction buffer [10X] composition:
330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium acetate, 5.0 mM DTT.
Storage Buffer:
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC
Storage Temperature:
-20ºC
Unit Definition:
One unit of T5 Exonuclease catalyzes the release of 1 nmol of acid-soluble nucleotides from double-stranded calf thymus DNA in 30 minutes at 37°C under standard assay conditions.
T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA. The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5´→3´ exonuclease activity of E. coli DNA Polymerase I.
Product Source:
Product Includes:
Applications:
T5 Exonuclease reaction buffer [10X] composition:
330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium acetate, 5.0 mM DTT.
Storage Buffer:
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC
Storage Temperature:
-20ºC
Unit Definition:
One unit of T5 Exonuclease catalyzes the release of 1 nmol of acid-soluble nucleotides from double-stranded calf thymus DNA in 30 minutes at 37°C under standard assay conditions.
