OrgoSol DetergentOUT™ (4 Citations)
OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. OrgoSol DetergentOUT™ is compatible with all detergent types and its performance is not dependent on the concentration of detergents in the solution. OrgoSol DetergentOUT™ is highly flexible and can be used to process small and large sample volumes.
OrgoSol DetergentOUT™ first concentrates the protein solution through precipitation and then the detergent is extracted and removed with the supplied OrgoSol™ buffer. The proprietary precipitation agent ensures >99% protein recovery, however precipitation may result in some loss of a protein's biological activity.
Two sizes are offered for single or multiple experiments:
Micro Kit for processing up to a total of 10ml protein solution
Medi Kit for processing up to a total of 30ml protein solution
|Material Safety Data Sheet|
|Concentration of Dilute Protein Solutions|
|Detergent Handbook & Selection Guide||A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.|
|Highly efficient detergent removal for improved mass spectrometry analysis||Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionization when analyzed by mass spectrometry. Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g. SDS, Triton® X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss for downstream analysis by mass spectrometry and other techniques. Remove Free Aqueous Detergents Prevent Interference of Downstream Applications Improve Mass Spec. Detection The application note shows how DetergentOUT™ GBS10 improves mass spec detection of peptides after detergent removal. Download the free application note to find the key to improved mass spectrometry analysis!|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|
|Plant Proteomics Handbook|
|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|
|Protein Purification Handbook|
|Removing Detergents from Protein Solutions|
|Sample Preparation Handbook||For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment|