PEROXsay™ Peroxide Assays

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the PEROX^say™ assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

 

PEROXsay™ is suitable for quantifying the level of protein damaging peroxides in biological solutions, measurement of lipid peroxidation of low density lipoproteins and liposomes and monitoring protein glycation.

 

The PEROXsay™ assay is designed for microtiter plates, but can be scaled up for use with 1ml cuvettes.

 

PEROXsay™-LIPID permits the quantification of peroxides in the presence of lipids without the need for extraction steps.