| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-325 | ProteSEEKER™ | 50 Assays | $567.00 |
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay.
ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.
ProteSEEKER™ protease screening assay consists of a ready-to-use dye-labeled protein, which is digested by proteases to release dye-labeled peptides. The absorbance of which is measured for determination of protease activity. The inhibitors are supplied at a 100X concentration and the 1X concentration provides >90% inhibition in most biological samples.
Protocols.io also provides an interactive step-by-step version of this protocol where you can discover and share optimizations with the research community.
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay.
ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.
ProteSEEKER™ protease screening assay consists of a ready-to-use dye-labeled protein, which is digested by proteases to release dye-labeled peptides. The absorbance of which is measured for determination of protease activity. The inhibitors are supplied at a 100X concentration and the 1X concentration provides >90% inhibition in most biological samples.
Protocols.io also provides an interactive step-by-step version of this protocol where you can discover and share optimizations with the research community.
