Hydroxylamine•HCl is a reducing agent that is routinely used for the deacetylation of SATA to form free sulfhydryls (Figure 1), for cleavage of protein cross-linkers that contain carbonyl groups (i.e. EGS) and for mutagenesis of plasmid DNA.
Features
- Synonym: Hydroxylammonium chloride
- Linear formula: NH2OH•HCl
- CAS #. 5470-11-1
- Molecular weight: 69.49
- Form: White crystalline powder
Applications
- Expose protected sulfhydryl groups on SAT-modified molecules
- Cleave carbonyl containing crosslinkers, such as EGS (Ref. 1)
- Random mutagenesis of plasmid DNA for plasmid shuffle or screening for ts mutants (Ref. 2)
