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TCEP-HCl; Tris (2-carboxyethyl) phosphine-HCl




TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applications. The reduction potency of TCEP is twice as high as that of DTT, and TCEP is effective in reducing proteins over a wider range of pH conditions, including lower acidic pH ranges (pH 2-11), compared to other reductants.

Since TCEP is a charged molecule, it cannot penetrate hydrophobic environments. Therefore, the use of a denaturant, acidic condition (pH 2.8-5) or basic condition (pH 9-11), is recommended for effective reduction.

TCEP reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions.

For more reducing agents, click here. for TCEP Reducing Gel


Features

  • Chemical Name: tris(2-carboxyethyl)phosphine, HCl
  • Synonym: TCEP, Hydrochloride
  • CAS Number: 51805-45-9
  • Chemical Formula: C9H15O6P•HCl
  • Molecular Weight: 286.65
  • Form: White crystalline solid
  • Solubility: H2O (5mg/ml)

 


Applications

  • Reduction of protein and peptide solutions

 

 

Material Safety Data Sheet
786-030
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786-873
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-874
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-875
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Protein Cross-Linking Handbook  A detailed guide to protein cross-linkers and their reactions.
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
Certificate Of Analysis
786-030
Lot 182012
Lot 182305
Lot 162904
786-873
182012
786-874
Lot 182012

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