RBC Lysis Buffer (17 Citations)

The ready-to-use, sterile RBC Lysis Buffer, Red Blood Cell Lysis Buffer, has been designed, formulated and tested for the optimal lysis of RBCs (erythrocytes), while having minimal effect of leukocytes, including lymphocytes, when used as directed. The RBC Lysis Buffer uses an optimized concentration of ammonium chloride and enhancers to lyze the red bloods cells that does not effectively lyze the nucleated RBCs. The use of RBC Lysis Buffer allows for the preferential lysis of red blood cells from whole blood and as these are the majority of cells in whole blood permits the concentration of the nucleated white blood cells.  Ideal for the isolation of DNA and RNA from blood. Using RBC Lysis Buffer eliminates the need for toxic organic solvents or chaotropes. Available in 100, 250, 500 and 1,000ml bottles.

Our RBC Lysis buffer is used in our XIT™ DNA from Whole Blood kits.

A 10X concentrated RBC Lysis Buffer is also available in 100 and 500ml sizes.

Features

• Optimal lysis of red blood cells
• Maintain white blood cells

Applications

• Removal of red blood cells
• Concentrate white blood cells for DNA and RNA isolation