RIPA Lysis and Extraction Buffer (97 Citations)
Catalog Numbers:
786-489 RIPA Lysis & Extraction Buffer 100ml
786-490 RIPA Lysis & Extraction Buffer 500ml
786-723 RIPA Lysis & Extraction Buffer 1L
786-746 RIPA Lysis & Extraction Buffer 1 Gallon
RIPA Lysis and Extraction Buffer 564
Catalog
Description
Size
Price(USD)
Qty
Catalog
786-489
786-489
Description
RIPA Lysis & Extraction Buffer
RIPA Lysis & Extraction Buffer
Size
100ml
100ml
$92.00
$92.00
Catalog
786-490
786-490
Description
RIPA Lysis & Extraction Buffer
RIPA Lysis & Extraction Buffer
Size
500ml
500ml
$200.00
$200.00
Catalog
786-723
786-723
Description
RIPA Lysis & Extraction Buffer
RIPA Lysis & Extraction Buffer
Size
1L
1L
$332.00
$332.00
Catalog
786-746
786-746
Description
RIPA Lysis & Extraction Buffer
RIPA Lysis & Extraction Buffer
Size
1 Gallon
1 Gallon
$1,020.00
$1,020.00
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.
RIPA Lysis Buffer does not contain protease inhibitors, however it is fully compatible with our range of individual protease inhibitors and cocktails.
Features
- 25 mM Tris, 150 mM Sodium Chloride, 1% NP-40, 1% Sodium Deoxycholate, 0.1% SDS, pH 7.6
| Protocol | |
| 786-489 | |
| 786-490 | |
| 786-723 | |
| 786-746 | |
| Material Safety Data Sheet | |
| 786-489 | |
| 786-490 | |
| 786-723 | |
| 786-746 | |
| Technical Literature | |
| Mass Spectrometry Sample Prep Handbook | A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation. |
| Protease & Phosphatase Inhibitors & Proteases Handbook | A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays |
| Protein Electrophoresis Handbook | A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products. |
| Sample Preparation Handbook | For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment |
| Western Blotting Handbook | From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins. |
| Certificate Of Analysis | |
| 786-489 | |
| 786-490 | |
| 786-723 | |
- Apoorva, Astha Shukla. et al. (2025) Proteome profiling of human lung epithelial cells unveils distinct patterns of protein expression in response to SARS-CoV-2 ORF3a, The Microbe, Volume 6, 2025, 100237, ISSN 2950-1946, https://doi.org/10.1016/j.microb.2025.100237.
- Bhadury, R. et al (2025) Development and Validation of AAV-Mediated Liver, Liver-VAT, and Liver-Brain SORT and Therapeutic Regulation of FASN in Hepatic De Novo Lipogenesis. Cells, Volume 14, Issue 5. https://doi.org/10.3390/cells14050372
- Hao, Shoujin et al. (2025). TNF inhibits NKCC2 phosphorylation by a calcineurin-dependent pathway. American Journal of Physiology-Renal Physiology vol 328 ISSN 4. doi: 10.1152/ajprenal.00251.2024
- Thakkar KJ, et al. (2025). Unconventional interaction of the zebrafish thrombopoietin receptor with Mapk14a determines thrombocyte development and function. J Thromb Haemost. S1538-7836(25)00146-1. doi: 10.1016/j.jtha.2025.03.004. Epub ahead of print. PMID: 40089183.
- Apoorva, A. et al (2024) Dengue virus NS1 hits hard at the barrier integrity of human cerebral microvascular endothelial cells via cellular microRNA dysregulations. Tissue Barriers. https://doi.org/10.1080/21688370.2024.2424628
- Choi, G. et al. (2024). The Inhibition Effect and Mechanism of Staurosporine Isolated from Streptomyces sp. SNC087 Strain on Nasal Polyp. Mar. Drugs , 22(1), 39; https://doi.org/10.3390/md22010039
- Colella, P. et al (2024) CNS-wide repopulation by hematopoieticderived microglia-like cells corrects progranulin deficiency in mice. Nature Communications, Volume 15, Article number 5654. https://doi.org/10.1038/s41467-024-49908-4.
- Janes ME et al. (2024). Dendritic Cell Immune Modulation via Polyphenol Membrane Coatings. ACS Appl Mater Interfaces. 16(22):28070-28079. doi: 10.1021/acsami.4c01575.
- Janes ME. et al. (2024) Dendritic Cell Immune Modulation via Polyphenol Membrane Coatings. ACS Appl Mater Interfaces. 2024 Jun 5;16(22):28070-28079. doi: 10.1021/acsami.4c01575.
- Joshi, P. et al (2024) Targeting host inducible-heat shock protein 70 with PES-Cl is a promising antiviral strategy against SARS-CoV-2 infection and pathogenesis. Volume 279, Part 1. https://doi.org/10.1016/j.ijbiomac.2024.135069
- Kim H. et al. (2024). Pericytes contribute to pulmonary vascular remodeling via HIF2α signaling. EMBO Rep. 25:616-645. doi: 10.1038/s44319-023-00054-w.
- Kour, R. et al (2024) Cassia fistula L. bark fraction modulated GSK3β/ p53 expression for mitochondrial mediated apoptosis in HeLa cells. South African Journal of Botany. Volume 168. https://doi.org/10.1016/j.sajb.2024.03.018
- Mai, D. et al. (2024). ZFP36 disruption is insufficient to enhance the function of mesothelin-targeting human CAR-T cells. Sci Rep 14, 3113. https://doi.org/10.1038/s41598-024-53769-8
- Mittal, A. et al (2024) Ethanol with thioacetamide murine model of alcoholic liver disease identifies hepatic pathways as targets for the human disease. Annals of Hepatology, Volume 30, Issue 1. https://doi.org/10.1016/j.aohep.2024.101565
- Rana, A.K. et al. (2024). β-Hydroxybutyrate Improves the Redox Status, Cytokine Production and Phagocytic Potency of Glucose-Deprived HMC3 Human Microglia-like Cells. J Neuroimmune Pharmacol 19, 35 https://doi.org/10.1007/s11481-024-10139-5
- Rani, H. & Saini, N. (2024) MicroRNA-718 inhibits mitochondrial fusion and ameliorates IMQinduced psoriasis inflammation mediated by PHB and STAT1. Research Square. https://doi.org/10.21203/rs.3.rs-4557621/v1
- Sarkar S. et al. (2024). Whole-body exposure to filtered fraction of diesel exhaust induced localized testicular damage through attenuated functional response of glutathione-s-transferase in adult male Wistar rats. Reprod Toxicol. 129:108682. doi: 10.1016/j.reprotox.2024.108682.
- Solheim, E.T. et al. (2024) Multi-omics profiling reveals dysregulated ribosome biogenesis and impaired cell proliferation following knockout of CDR2L. BMC Cancer 24, 645. https://doi.org/10.1186/s12885-024-12399-z
- Talukdar, S. et al (2024) Mycobacterium tuberculosis exploits SIRT2 for iron acquisition to facilitate its intracellular survival. BioRxiv. https://doi.org/10.1101/2024.01.05.574348
- Yang D. et al. (2024). Phosphorylation of pyruvate dehydrogenase inversely associates with neuronal activity. Neuron. 112:959-971.e8. doi: 10.1016/j.neuron.2023.12.015.
- Adaku, N., et al. (2023). Apolipoprotein E2 stimulates protein synthesis and promotes melanoma progression and metastasis. Cancer Research, 83(18), 3013–3025. https://doi.org/10.1158/0008-5472.can-23-1252
- Bhandari, N. et al. (2023). Pan-cancer integrated bioinformatic analysis of RNA polymerase subunits reveal RNA Pol I member CD3EAP regulates cell growth by modulating autophagy. Cell Cycle, 22(18), 1986–2002. https://doi.org/10.1080/15384101.2023.2265676
- Bhardwaj, Utkarsh & Singh, Sunit (2023) Zika virus NS1 suppresses VE-cadherin via hsa-miR-29b-3p/DNMT3b/MMP-9 pathway in human brain microvascular endothelial cells. CELL SIGNAL. https://doi.org/10.1016/j.cellsig.2023.110659
- Cerqueira, A. et al (2023) Proteomic Analysis of Mesenchymal Stem Cells and Monocyte Co-Cultures Exposed to a Bioactive Silica-Based Sol–Gel Coating. ACS Biomaterials Science & Engineering, Volume 9, Issue 6, page 3307. https://doi.org/10.1021/acsbiomaterials.3c00254
- Cerqueira, A. et al (2023) Proteomic Analysis of Mesenchymal Stem Cells and Monocyte Co-Cultures Exposed to a Bioactive Silica-Based Sol–Gel Coating. ACS Biomaterials Science & Engineering DOI: 10.1021/acsbiomaterials.3c00254
- Joesph, A., et al. (2023) Intrauterine colonization with Gardnerella vaginalis and Mobiluncus mulieris induces maternal inflammation but not preterm birth in a mouse model Am J Reprod Immunol. 2023 Aug;90(2):e13749. doi: 10.1111/aji.13749.
- Mai, David et al (2023) Combined disruption of T cell inflammatory regulators regnase-1 and roquin-1 enhances antitumor activity of engineered human T cells. PNAS. https://doi.org/10.1073/pnas.2218632120
- Modi, J. et al (2023) The Role of NMDA Receptor Partial Antagonist, Carbamathione, as a Therapeutic Agent for Transient Global Ischemia. Biomedicines. Volume 11, Issue 7. https://doi.org/10.3390/biomedicines11071885
- Montero-Calle, A et al (2023) Benefits of FAIMS to Improve the Proteome Coverage of Deteriorated and/or Cross-Linked TMT 10-Plex FFPE Tissue and Plasma-Derived Exosomes Samples. Proteomes.
- Ortigosa-Palomo A, et al. (2023) Antitumor activity of bengamide ii in a panel of human and murine tumor cell lines: In vitro and in vivo determination of effectiveness against lung cancer. Biomed Pharmacother. Dec;168:115789. doi: 10.1016/j.biopha.2023.115789. Epub 2023 Nov 2. PMID: 37924787.
- Pandey, N., & Singh, S. K. (2023). MicroRNA-155 triggers a cellular antiviral immune response against Chandipura virus in human microglial cells. Microbes and Infection, 25(7), 105173. https://doi.org/10.1016/j.micinf.2023.105175
- Parmar, F. et al (2023) Anti-proliferative efficacy of ALSTONIA SCHOLARIS (L.) R.Br. extraction on cultured Daudi cells: An in Vitro Approach. Vidya Journal. Volume 2, Issue 2: Page 28.
- Pérez-Montes, C. et al (2023). REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration. Antioxidants, 12(12), 2026. https://doi.org/10.3390/antiox12122026
- Richards, T. et al (2023) Therapeutic Intervention of Neuroinflammatory Alzheimer Disease Model by Inhibition of Classical Complement Pathway with the Use of Anti-C1r Loaded Exosomes. Research Square. Page 9.
- Shukla, A. et al. (2023) Hypoxia-Induced miR-101 Impairs Endothelial Barrier Integrity Through Altering VE-Cadherin and Claudin-5. Mol Neurobiol 61, 1807–1817. https://doi.org/10.1007/s12035-023-03662-8
- Singh, A. et al (2023) Polyethylene glycol-based isolation of urinary extracellular vesicles, an easily adoptable protocol. MethodsX. Volume 11: Pages 4-6. https://doi.org/10.1016/j.mex.2023.102310
- Singh, A. et al (2023). Polyethylene glycol-based isolation of urinary extracellular vesicles, an easily adoptable protocol. MethodsX vol 11. https://doi.org/10.1016/j.mex.2023.102310.
- Vishwakarma, V.K. et al (2023) Effect of vinpocetine alone and in combination with enalapril in experimental model of diabetic cardiomyopathy in rats: possible involvement of PDE-1/TGF-β/ Smad 2/3 signalling pathways. Journal of Pharmacy and Pharmacology, Volume 75, Issue 9, Pages 1198–1211. https://doi.org/10.1093/jpp/rgad043
- Yang, Dong et al (2023) Phosphorylation of pyruvate dehydrogenase marks the inhibition of in vivo neuronal activity. BIORXIV. https://doi.org/10.1101/2023.03.13.532494
- Farnsworth, D.A. et al (2022) MEK inhibitor resistance in lung adenocarcinoma is associated with addiction to sustained ERK suppression. NPJ PRECIS. https://doi.org/10.1038/s41698-022-00328-x
- Koya, Jagadish B. et al (2022) FDA-approved excipient N, N-Dimethylacetamide attenuates inflammatory bowel disease in In Vitro and In Vivo models. FORTUNE. https://doi.org/10.26502/fjhs.076
- Lee, Sanghoon et al (2022) Mechanism of paracrine communications between hepatic progenitor cells and endothelial cells. CELL SIGNAL. https://doi.org/10.1016/j.cellsig.2022.110458
- Moy, Ryan H. et al (2022) Functional genetic screen identifies ITPR2/calcium/RELB axis as a driver of colorectal cancer metastic liver colonization. DEV CELL. https://doi.org/10.1016/j.devcel.2022.04.010
- Wang, Y. et al (2022) The role of somatosensory innervation of adipose tissues. NATURE. https://doi.org/10.1038/s41586-022-05137-7
- Wei, Zeng-Hui et al (2022) N,N-Dimethylacetamide targets neuroinflammation in Alzheimer's disease in in-vitro and ex-vivo models. RESEARCH SQUARE. https://doi.org/10.21203/rs.3.rs-2363746/v1
- Ayushi, C. et al (2021) Pathogen induced subversion of NAD+ metabolism mediating host cell death: a target for development of chemotherapeutics. Cell Death Discov. doi.org/10.1038/s41420-020-00366-z
- Bhardwaj, Utkarsh & Singh, Sunit K. (2021) Zika Virus NS1 Suppresses VE-Cadherin and Claudin-5 via hsa-miR-101-3p in Human Brain Microvascular Endothelial Cells. MOL NEUROBIOL. https://doi.org/10.1007/s12035-021-02548-x
- Koya, Jagadish et al (2021) FCA-Approved Excipient N,N-Dimethylacetamide Attenuates Inflammatory Bowel Disease in In-Vitro and In-Vivo Models. RESEARCH SQUARE. https://doi.org/10.21203/rs.3.rs-948100/v1
- Malabika, et al. (2021) Scientific Reports (Nature Publisher Group) 11:1 DOI:10.1038/s41598-021-83513-5
- Shukla, Astha et al (2021) Zika virus NS1 suppresses the innate immune responses via miR-146a in human microglial cells. INT J BIOL MACROMOL. https://doi.org/10.1016/j.ijbiomac.2021.11.061
- Buriachenko, SV (2020) International Scientific and Practical Conferance. doi.org/10.30525/978-9934-588-81-5-2.30
- Lu, H and Kang, F (2020) BioS Rep. doi.org/10.1042/BSR20201373
- Rachmi, E. et al (2020) Afzelin inhibits migration of MDA-MB-231 cells by suppressing FAK expression and Rac1 activation. J Appl Pharm Sci. DOI: 10.7324/JAPS.2020.101010.
- Chao, S. et al (2019) Glucosamine Enhances TRAIL-Induced Apoptosis in the Prostate Cancer Cell Line DU145. Medicines. 6(4):104
- Cultrara, C. et al (2019) Size Matters: Arginine-Derived Peptides Targeting the PSMA Receptor can Efficiently Complex but not Transfect siRNA. Molecular Therapy-Nucleic Acids. 10.1016/j.omtn.2019.10.013
- Dolinko, A. H. et al (2019) African and Asian Mitochondrial DNA Haplogroups Confer Resistance Against Diabetic Stresses on Retinal Pigment Epithelial Cybrid Cells In Vitro. Biol Futur.doi.org/10.1007/s12035-019-01834-z.
- Jiao, Wu. et al (2019) Signal regulatory protein alpha initiates cachexia through muscle to adipose tissue crosstalk. J CACHEXIA SARCOPEN. 10: 1210–1227
- Manuel, C. R. et al (2019) ImmunMe tolerance attenuates gut dysbiosis, dysregulated uterine gene expression and high-fat diet potentiated preterm birth in mice. Am. J. Obstet. Gynecol.doi.org/10.1016/j.ajog.2019.02.028
- Sun, C. Glucosamine Enhances TRAIL-Induced Apoptosis in the Prostate Cancer Cell Line DU145. MEDICINE. 6(4):104
- Wu, J. et al (2019) Signal regulatory protein alpha initiates cachexia through muscle to adipose tissue crosstalk. J CACHEXIA SARCOPENI. 10:1210–1227
- Manuel,C.R. (2018) Immune Tolerance attenuates High-Fat Diet Potentiated Preterm Birth.
- Shivakumar, S. et al (2018) Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux.Biomed Res Int.doi.org/10.1155/2018/7627329
- Vora, A.N. et al(2018)Molecular candidates for Blocking the Transmission of Vector-Borne diseases. DOI: 10.25777/qa8c-kk58.
- Mandal, K. et al (2017) An integrated transcriptomics-guided genome-wide promoter analysis and next-generation proteomics approach to mine factor(s) regulating cellular differentiation. DOI: https://doi.org/10.1093/dnares/dsw057
- Pepping J.K. et al (2017) Myeloid-specific deletion of NOX2 prevents the metabolic and neurologic consequences of high fat diet. PLoS One. https://doi.org/10.1371/journal.pone.0181500
- Rhee, C. et al (2017) Mechanisms of transcription factor-mediated direct reprogramming of mouse embryonic stem cells to trophoblast stem-like cells. Nucleic Acids Research. https://doi.org/10.1093/nar/gkx692
- Sharma, S. S. and Mujumdar, S.S. (2017) Transcriptional co-activator YAP regulates cAMP signaling in Sertoli cells. Mol Cell Endocrinol. https://doi.org/10.1016/j.mce.2017.04.017
- Shetty, R. et al (2017) Oxidative stress induces dysregulated autophagy in corneal epithelium of keratoconus patients. PLOS One. https://doi.org/10.1371/journal.pone.0184628
- Boakye, C. H. et al (2016) Ultra-flexible nanocarriers for enhanced topical delivery of a highly lipophilic antioxidative molecule for skin cancer chemoprevention. Colloids Surf B Biointerfaces.doi:10.1016/j.colsurfb.2016.03.036
- Boakye, C.H.A. et al ( 2016) Novel amphiphilic lipid augments the co-delivery of erlotinib and IL36 siRNA into the skin for psoriasis treatment. J Control Release. doi:10.1016/j.jconrel.2016.05.017
- Carbrera, A.P. et al (2016) Senescence Increases Choroidal Endothelial Stiffness and Susceptibility to Complement Injury: Implications for Choriocapillaris Loss in AMD. IOVS doi:10.1167/iovs.16-19727
- Haroon, M. M. et al (2016) A designed recombinant fusion protein for targeted delivery of siRNA to the mouse brain. J Control Release.228:120
- Messeha,S.S at al (2016)The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A . European J Med Plants. DOI: 10.9734/EJMP/2016/23992.
- Tsai, P. et al (2016)Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo. Molecules. 21:1
- Vlaminck, J. et al (2016) Community Rates of IgG4 Antibodies to Ascaris Haemoglobin Reflect Changes in Community Egg Loads Following Mass Drug Administration. PLoS Negl Trop Dis. doi.org/10.1371/journal.pntd.0004532
- Hagan, S. et al (2015) Andrology, DOI: 10.1111/andr.12074
- Kim, H. R. et al (2015) Toxicology Letters. 233:148
- Patel, S. et al (2015) Cell. Oncol. DOI: 10.1007/s13402-015-0235-7
- Ramesh, A. et al (2015) Mol. Cell. Biochem. DOI: 10.1007/s11010-015-2600-2
- Sharma, A. et al (2015) Chikungunya Virus Infection Alters Expression of MicroRNAs Involved in Cellular Proliferation, Immune Response and Apoptosis. Intervirology. 58:332
- Sutherland, J.M. et al (2015) Biomolecules. 5(3):1228
- Boakye, C. H. et al (2014) J. Biomed. Nanotechnol. 11:1269
- Keegan, K. et al (2014) Mol. Cancer Ther. 13:880
- Marepally, S. et al (2014) Nanomedicine. 9:2157
- Ranade, S. S. R. et al (2014) PNAS. 111:10347
- Reschke, M. E. et al (2014) BBA. Proteins and proteomics. 1844:2182
- Andey, T. et al (2013) Eur. J. Pharma. Sci. 50:227
- Arany, S. et al (2013)Mol. Therapy. 21:1182
- Desai. P.R. et al (2013) J. Control. Release. 170:51
- Higashikuni, Y et al (2013) JAHA. 2: e000267
- Lee, A.B. et al (2013) Nature. 493:416
- Pepping, J.K. et al (2013) Am J Physiol Endocrinol Metab. 304:E392
- Arany, S. et al (2012) J. Cell. Biochem. 113:1955
- Kahle, M.P. et al (2012) Neuroreport. 23:627
- McNulty, S.N. et al (2012) PLOS. 7(9): e45777
- Wu, D. et al (2012) J. Clin. Invest. 122:1306
- Zhang, L. et al (2011) Am J Physiol Endocrinol Metab 301:E599