RIPA Lysis and Extraction Buffer (63 Citations)

Catalog
Description
Size
Price(USD)
Qty
Catalog
786-489
786-489
Description
RIPA Lysis & Extraction Buffer
RIPA Lysis & Extraction Buffer
Size
100ml
100ml
$87.00
$87.00
Catalog
786-490
786-490
Description
RIPA Lysis & Extraction Buffer
RIPA Lysis & Extraction Buffer
Size
500ml
500ml
$190.00
$190.00
Catalog
786-723
786-723
Description
RIPA Lysis & Extraction Buffer
RIPA Lysis & Extraction Buffer
Size
1L
1L
$316.00
$316.00
Catalog
786-746
786-746
Description
RIPA Lysis & Extraction Buffer
RIPA Lysis & Extraction Buffer
Size
1 Gallon
1 Gallon
$971.00
$971.00
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.
RIPA Lysis Buffer does not contain protease inhibitors, however it is fully compatible with our range of individual protease inhibitors and cocktails.
Features
- 25 mM Tris, 150 mM Sodium Chloride, 1% NP-40, 1% Sodium Deoxycholate, 0.1% SDS, pH 7.6
Protocol | |
786-489 | ![]() |
786-490 | ![]() |
786-723 | ![]() |
786-746 | ![]() |
Material Safety Data Sheet | |
786-489 | |
786-490 | |
786-723 | |
786-746 |
Technical Literature | |
Mass Spectrometry Sample Prep Handbook | A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation. |
Protease & Phosphatase Inhibitors & Proteases Handbook | A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays |
Protein Electrophoresis Handbook | A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products. |
Sample Preparation Handbook | For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment |
Western Blotting Handbook | From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins. |
Certificate Of Analysis | |
786-489 | |
786-490 | |
786-723 |
- Bhardwaj, Utkarsh & Singh, Sunit (2023) Zika virus NS1 suppresses VE-cadherin via hsa-miR-29b-3p/DNMT3b/MMP-9 pathway in human brain microvascular endothelial cells. CELL SIGNAL. https://doi.org/10.1016/j.cellsig.2023.110659
- Cerqueira, A. et al (2023) Proteomic Analysis of Mesenchymal Stem Cells and Monocyte Co-Cultures Exposed to a Bioactive Silica-Based Sol–Gel Coating. ACS Biomaterials Science & Engineering DOI: 10.1021/acsbiomaterials.3c00254
- Mai, David et al (2023) Combined disruption of T cell inflammatory regulators regnase-1 and roquin-1 enhances antitumor activity of engineered human T cells. PNAS. https://doi.org/10.1073/pnas.2218632120
- Montero-Calle, A et al (2023) Benefits of FAIMS to Improve the Proteome Coverage of Deteriorated and/or Cross-Linked TMT 10-Plex FFPE Tissue and Plasma-Derived Exosomes Samples. Proteomes.
- Yang, Dong et al (2023) Phosphorylation of pyruvate dehydrogenase marks the inhibition of in vivo neuronal activity. BIORXIV. https://doi.org/10.1101/2023.03.13.532494
- Farnsworth, D.A. et al (2022) MEK inhibitor resistance in lung adenocarcinoma is associated with addiction to sustained ERK suppression. NPJ PRECIS. https://doi.org/10.1038/s41698-022-00328-x
- Koya, Jagadish B. et al (2022) FDA-approved excipient N, N-Dimethylacetamide attenuates inflammatory bowel disease in In Vitro and In Vivo models. FORTUNE. https://doi.org/10.26502/fjhs.076
- Lee, Sanghoon et al (2022) Mechanism of paracrine communications between hepatic progenitor cells and endothelial cells. CELL SIGNAL. https://doi.org/10.1016/j.cellsig.2022.110458
- Moy, Ryan H. et al (2022) Functional genetic screen identifies ITPR2/calcium/RELB axis as a driver of colorectal cancer metastic liver colonization. DEV CELL. https://doi.org/10.1016/j.devcel.2022.04.010
- Wang, Y. et al (2022) The role of somatosensory innervation of adipose tissues. NATURE. https://doi.org/10.1038/s41586-022-05137-7
- Wei, Zeng-Hui et al (2022) N,N-Dimethylacetamide targets neuroinflammation in Alzheimer's disease in in-vitro and ex-vivo models. RESEARCH SQUARE. https://doi.org/10.21203/rs.3.rs-2363746/v1
- Ayushi, C. et al (2021) Pathogen induced subversion of NAD+ metabolism mediating host cell death: a target for development of chemotherapeutics. Cell Death Discov. doi.org/10.1038/s41420-020-00366-z
- Bhardwaj, Utkarsh & Singh, Sunit K. (2021) Zika Virus NS1 Suppresses VE-Cadherin and Claudin-5 via hsa-miR-101-3p in Human Brain Microvascular Endothelial Cells. MOL NEUROBIOL. https://doi.org/10.1007/s12035-021-02548-x
- Koya, Jagadish et al (2021) FCA-Approved Excipient N,N-Dimethylacetamide Attenuates Inflammatory Bowel Disease in In-Vitro and In-Vivo Models. RESEARCH SQUARE. https://doi.org/10.21203/rs.3.rs-948100/v1
- Malabika, et al. (2021) Scientific Reports (Nature Publisher Group) 11:1 DOI:10.1038/s41598-021-83513-5
- Shukla, Astha et al (2021) Zika virus NS1 suppresses the innate immune responses via miR-146a in human microglial cells. INT J BIOL MACROMOL. https://doi.org/10.1016/j.ijbiomac.2021.11.061
- Buriachenko, SV (2020) International Scientific and Practical Conferance. doi.org/10.30525/978-9934-588-81-5-2.30
- Lu, H and Kang, F (2020) BioS Rep. doi.org/10.1042/BSR20201373
- Rachmi, E. et al (2020) Afzelin inhibits migration of MDA-MB-231 cells by suppressing FAK expression and Rac1 activation. J Appl Pharm Sci. DOI: 10.7324/JAPS.2020.101010.
- Chao, S. et al (2019) Glucosamine Enhances TRAIL-Induced Apoptosis in the Prostate Cancer Cell Line DU145. Medicines. 6(4):104
- Cultrara, C. et al (2019) Size Matters: Arginine-Derived Peptides Targeting the PSMA Receptor can Efficiently Complex but not Transfect siRNA. Molecular Therapy-Nucleic Acids. 10.1016/j.omtn.2019.10.013
- Dolinko, A. H. et al (2019) African and Asian Mitochondrial DNA Haplogroups Confer Resistance Against Diabetic Stresses on Retinal Pigment Epithelial Cybrid Cells In Vitro. Biol Futur.doi.org/10.1007/s12035-019-01834-z.
- Jiao, Wu. et al (2019) Signal regulatory protein alpha initiates cachexia through muscle to adipose tissue crosstalk. J CACHEXIA SARCOPEN. 10: 1210–1227
- Manuel, C. R. et al (2019) ImmunMe tolerance attenuates gut dysbiosis, dysregulated uterine gene expression and high-fat diet potentiated preterm birth in mice. Am. J. Obstet. Gynecol.doi.org/10.1016/j.ajog.2019.02.028
- Sun, C. Glucosamine Enhances TRAIL-Induced Apoptosis in the Prostate Cancer Cell Line DU145. MEDICINE. 6(4):104
- Wu, J. et al (2019) Signal regulatory protein alpha initiates cachexia through muscle to adipose tissue crosstalk. J CACHEXIA SARCOPENI. 10:1210–1227
- Manuel,C.R. (2018) Immune Tolerance attenuates High-Fat Diet Potentiated Preterm Birth.
- Shivakumar, S. et al (2018) Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux.Biomed Res Int.doi.org/10.1155/2018/7627329
- Vora, A.N. et al(2018)Molecular candidates for Blocking the Transmission of Vector-Borne diseases. DOI: 10.25777/qa8c-kk58.
- Mandal, K. et al (2017) An integrated transcriptomics-guided genome-wide promoter analysis and next-generation proteomics approach to mine factor(s) regulating cellular differentiation. DOI: https://doi.org/10.1093/dnares/dsw057
- Pepping J.K. et al (2017) Myeloid-specific deletion of NOX2 prevents the metabolic and neurologic consequences of high fat diet. PLoS One. https://doi.org/10.1371/journal.pone.0181500
- Rhee, C. et al (2017) Mechanisms of transcription factor-mediated direct reprogramming of mouse embryonic stem cells to trophoblast stem-like cells. Nucleic Acids Research. https://doi.org/10.1093/nar/gkx692
- Sharma, S. S. and Mujumdar, S.S. (2017) Transcriptional co-activator YAP regulates cAMP signaling in Sertoli cells. Mol Cell Endocrinol. https://doi.org/10.1016/j.mce.2017.04.017
- Shetty, R. et al (2017) Oxidative stress induces dysregulated autophagy in corneal epithelium of keratoconus patients. PLOS One. https://doi.org/10.1371/journal.pone.0184628
- Boakye, C. H. et al (2016) Ultra-flexible nanocarriers for enhanced topical delivery of a highly lipophilic antioxidative molecule for skin cancer chemoprevention. Colloids Surf B Biointerfaces.doi:10.1016/j.colsurfb.2016.03.036
- Boakye, C.H.A. et al ( 2016) Novel amphiphilic lipid augments the co-delivery of erlotinib and IL36 siRNA into the skin for psoriasis treatment. J Control Release. doi:10.1016/j.jconrel.2016.05.017
- Carbrera, A.P. et al (2016) Senescence Increases Choroidal Endothelial Stiffness and Susceptibility to Complement Injury: Implications for Choriocapillaris Loss in AMD. IOVS doi:10.1167/iovs.16-19727
- Haroon, M. M. et al (2016) A designed recombinant fusion protein for targeted delivery of siRNA to the mouse brain. J Control Release.228:120
- Messeha,S.S at al (2016)The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A . European J Med Plants. DOI: 10.9734/EJMP/2016/23992.
- Tsai, P. et al (2016)Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo. Molecules. 21:1
- Vlaminck, J. et al (2016) Community Rates of IgG4 Antibodies to Ascaris Haemoglobin Reflect Changes in Community Egg Loads Following Mass Drug Administration. PLoS Negl Trop Dis. doi.org/10.1371/journal.pntd.0004532
- Hagan, S. et al (2015) Andrology, DOI: 10.1111/andr.12074
- Kim, H. R. et al (2015) Toxicology Letters. 233:148
- Patel, S. et al (2015) Cell. Oncol. DOI: 10.1007/s13402-015-0235-7
- Ramesh, A. et al (2015) Mol. Cell. Biochem. DOI: 10.1007/s11010-015-2600-2
- Sharma, A. et al (2015) Chikungunya Virus Infection Alters Expression of MicroRNAs Involved in Cellular Proliferation, Immune Response and Apoptosis. Intervirology. 58:332
- Sutherland, J.M. et al (2015) Biomolecules. 5(3):1228
- Boakye, C. H. et al (2014) J. Biomed. Nanotechnol. 11:1269
- Keegan, K. et al (2014) Mol. Cancer Ther. 13:880
- Marepally, S. et al (2014) Nanomedicine. 9:2157
- Ranade, S. S. R. et al (2014) PNAS. 111:10347
- Reschke, M. E. et al (2014) BBA. Proteins and proteomics. 1844:2182
- Andey, T. et al (2013) Eur. J. Pharma. Sci. 50:227
- Arany, S. et al (2013)Mol. Therapy. 21:1182
- Desai. P.R. et al (2013) J. Control. Release. 170:51
- Higashikuni, Y et al (2013) JAHA. 2: e000267
- Lee, A.B. et al (2013) Nature. 493:416
- Pepping, J.K. et al (2013) Am J Physiol Endocrinol Metab. 304:E392
- Arany, S. et al (2012) J. Cell. Biochem. 113:1955
- Kahle, M.P. et al (2012) Neuroreport. 23:627
- McNulty, S.N. et al (2012) PLOS. 7(9): e45777
- Wu, D. et al (2012) J. Clin. Invest. 122:1306
- Zhang, L. et al (2011) Am J Physiol Endocrinol Metab 301:E599