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Optimizer Buffer™

Optimizer Buffer™
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Optimizer Buffer™
Optimizer Buffer™
Catalog Description Size Price (USD) Qty
BKC-04Optimizer Buffer™-I [5X]2 x 25ml$126.00
BKC-05Optimizer Buffer™-II [5X]2 x 25ml$126.00
BKC-06Optimizer Buffer™-III [5X]2 x 25ml$126.00
BKC-07Optimizer Buffer™-IV [5X]2 x 25ml$126.00
BKC-08Optimizer Buffer™-V [5X]2 x 25ml$126.00
BKC-09Optimizer Buffer™-VI [5X]2 x 25ml$126.00
BKC-10Optimizer Buffer™-VII 2 x 125 ml$126.00
BKC-11Optimizer Buffer™-VIII [5X]2 x 25 ml$126.00
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  • Description
    The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions, for example Tris buffers inhibit coupling to amines.
     
    G-Biosciences has prepared six reaction specific buffers that provide the optimal conditions for protein labeling, modification, and cross reaction. The table below highlights the reaction each buffer is specific for:
     
    Optimizer Buffer™  Reaction Type  Reactive Group
    I  Amine & Photoreactive Reactions  NHS-ester & imidoester groups
    II  Sulfhydryl Reactions  Iodoacetyl groups
    III  Sulfhydryl Reactions  Maleimides & pyridyl sulfides
    IV  Carboxyl Reactions  Carbodiimides
    V
    		  Carbohydrate Reactions  Hydrazide groups
    VI  Amine Reactions  Glyoxal groups
    VII Maleimide Conjugation  Sulfhydryl group
    VIII Citraconic Modification  Primary amine
     
    These buffers contain optimized concentration of buffering agents, pH, and other cofactors for specific reactions. Simply exchange the buffer of your sample with a suitable Optimizer Buffer™ and you are ready for efficient reaction. Use of Tube-O-DIALYZER™ is recommended for buffer exchange and optimal reaction results.
  • Reviews
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions, for example Tris buffers inhibit coupling to amines.
 
G-Biosciences has prepared six reaction specific buffers that provide the optimal conditions for protein labeling, modification, and cross reaction. The table below highlights the reaction each buffer is specific for:
 
Optimizer Buffer™  Reaction Type  Reactive Group
I  Amine & Photoreactive Reactions  NHS-ester & imidoester groups
II  Sulfhydryl Reactions  Iodoacetyl groups
III  Sulfhydryl Reactions  Maleimides & pyridyl sulfides
IV  Carboxyl Reactions  Carbodiimides
V
  Carbohydrate Reactions  Hydrazide groups
VI  Amine Reactions  Glyoxal groups
VII Maleimide Conjugation  Sulfhydryl group
VIII Citraconic Modification  Primary amine
 
These buffers contain optimized concentration of buffering agents, pH, and other cofactors for specific reactions. Simply exchange the buffer of your sample with a suitable Optimizer Buffer™ and you are ready for efficient reaction. Use of Tube-O-DIALYZER™ is recommended for buffer exchange and optimal reaction results.

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