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SpinOUT™ Columns

SpinOUT™ Columns
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SpinOUT™ Columns
SpinOUT™ Columns
Catalog Description Size Price (USD) Qty
786-865SpinOUT™ GT-100, 0.1ml25 columns$159.00
786-866SpinOUT™ GT-100, 1ml10 columns$76.00
786-867SpinOUT™ GT-100, 3ml10 columns$158.00
786-897SpinOUT™ GT-100, 3ml5 x 10/bag$526.00
786-868SpinOUT™ GT-100, 5ml5 columns$185.00
786-898SpinOUT™ GT-100, 5ml50 columns$646.00
786-869SpinOUT™ GT-100, 10ml5 columns$305.00
786-703SpinOUT™ GT-600, 0.1ml25 columns$159.00
786-170SpinOUT™ GT-600, 1ml10 Columns$76.00
786-720SpinOUT™ GT-600, 1ml5 x 10/bag$379.00
786-171SpinOUT™ GT-600, 3ml10 Columns$122.00
786-721SpinOUT™ GT-600, 3ml5 x 10/bag$526.00
786-704SpinOUT™ GT-600, 5ml5 columns$99.00
786-722SpinOUT™ GT-600, 5ml50 columns$772.00
786-705SpinOUT™ GT-600, 10ml5 columns$199.00
786-989SpinOut™ GT-600 Spin Plate2 plates$318.00
786-990SpinOut™ GT-600 Spin Plate4 plates$572.00
786-706SpinOUT™ GT-1200, 0.1ml25 columns$159.00
786-172SpinOUT™ GT-1200, 1ml10 Columns$76.00
786-1821SpinOUT™ GT-1200, 0.5ml10 columns$76.00
786-173SpinOUT™ GT-1200, 3ml10 Columns$122.00
786-1822SpinOUT™ GT-1200, 2ml10 columns$101.00
786-707SpinOUT™ GT-1200, 5ml5 columns$111.00
786-708SpinOUT™ GT-1200, 10ml5 columns$152.00
786-991SpinOut™ GT-1200 Spin Plate2 plates$318.00
786-992SpinOut™ GT-1200 Spin Plate4 plates$576.00
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  • Description

    Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. 

     

    The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes. Also available in 96-well spin plate formats for processing up to 96 samples.
     
    The SpinOUT™ columns are available in three MWCO sizes for >700, >6,000 or >30,000 Dalton peptides or proteins and are suitable for samples containing as little as 20µg protein/ml.
     
    The SpinOUT™ columns are easy to use; simply apply the protein sample and centrifuge to recover proteins and nucleic acids with the column retaining more than 95% of the salts and small molecules (<100Da for SpinOUT™ GT-100, <1,000Da for SpinOUT™ GT-600 and <1,500Da for SpinOUT™ GT-1200).
     
    Spin-OUT™ GT-100 is for the purification of peptides and proteins >700Da.
     
    Spin-OUT™ GT-600 is for the purification of proteins >6kDa and nucleic acids larger than 10bp.
     
    Spin-OUT™ GT-1200 is for the purification of proteins >30kDa and removal of molecules >1,500Da. The columns are ideal for separating proteins from peptides.
     

    Five sizes are available for each MWCO and are highlighted below:

    Cat. # Description # Supplied Resin Bed Volume (mL) Sample Load Volume (mL)

    786-865
    SpinOUT™ GT-100, 0.1ml
    25
    0.1
    0.005-0.02
    786-866
    SpinOUT™ GT-100, 1ml

    10

    		 1
    0.05-0.1
    786-867
    SpinOUT™ GT-100, 3ml
    		10 3
    0.1-0.5
    786-868
    SpinOUT™ GT-100, 5ml
    		5 5
    0.5-2
    786-869
    SpinOUT™ GT-100, 10ml
    		5 10
    0.5-4
    786-703
    SpinOUT™ GT-600, 0.1ml
    		25 0.1
    0.005-0.02
    786-170
    SpinOUT™ GT-600, 1ml
    		10 1
    0.05-0.1
    786-171
    SpinOUT™ GT-600, 3ml
    		10 3
    0.1-0.5
    786-704
    SpinOUT™ GT-600, 5ml
    		5 5
    0.5-2
    786-705
    SpinOUT™ GT-600, 10ml
    		5 10
    0.5-4
    786-706
    SpinOUT™ GT-1200, 0.1ml
    		25 0.1
    0.005-0.02
    786-1821
    SpinOUT™ GT-1200, 0.5ml
    		10 0.5
    0.025-0.1
    786-172
    SpinOUT™ GT-1200, 1ml
    		10 1
    0.05-0.1
    786-1822
    SpinOUT™ GT-1200, 2ml
    		10 2
    0.1-0.2
    786-173
    SpinOUT™ GT-1200, 3ml
    		10 3
    0.1-0.5
    786-707
    SpinOUT™ GT-1200, 5ml
    		5 5
    0.5-2
    786-708
    SpinOUT™ GT-1200, 10ml
    		5 10
    0.5-4

     

    For the desalting and clean up of PCR reactions, click here.
     

     

     


    Features

    • Offered in 3 MWCO sizes: >700Da,  >6kDa and >30kDa proteins 
    • Available in 5 sizes for sample volumes of 5µl to 4ml
    • 96-well plate formats available
    • Easy-to-use, spin-format columns for rapid purification

     


    Applications

    • Protein purification and contamination removal from samples
    • For the desalting and buffer exchange of protein and nucleic acid solutions
    • Separate proteins from peptides
    • Desalt peptides 
  • Reviews

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. 

 

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes. Also available in 96-well spin plate formats for processing up to 96 samples.
 
The SpinOUT™ columns are available in three MWCO sizes for >700, >6,000 or >30,000 Dalton peptides or proteins and are suitable for samples containing as little as 20µg protein/ml.
 
The SpinOUT™ columns are easy to use; simply apply the protein sample and centrifuge to recover proteins and nucleic acids with the column retaining more than 95% of the salts and small molecules (<100Da for SpinOUT™ GT-100, <1,000Da for SpinOUT™ GT-600 and <1,500Da for SpinOUT™ GT-1200).
 
Spin-OUT™ GT-100 is for the purification of peptides and proteins >700Da.
 
Spin-OUT™ GT-600 is for the purification of proteins >6kDa and nucleic acids larger than 10bp.
 
Spin-OUT™ GT-1200 is for the purification of proteins >30kDa and removal of molecules >1,500Da. The columns are ideal for separating proteins from peptides.
 

Five sizes are available for each MWCO and are highlighted below:

Cat. # Description # Supplied Resin Bed Volume (mL) Sample Load Volume (mL)

786-865
SpinOUT™ GT-100, 0.1ml
25
0.1
0.005-0.02
786-866
SpinOUT™ GT-100, 1ml

10

 1
0.05-0.1
786-867
SpinOUT™ GT-100, 3ml
10 3
0.1-0.5
786-868
SpinOUT™ GT-100, 5ml
5 5
0.5-2
786-869
SpinOUT™ GT-100, 10ml
5 10
0.5-4
786-703
SpinOUT™ GT-600, 0.1ml
25 0.1
0.005-0.02
786-170
SpinOUT™ GT-600, 1ml
10 1
0.05-0.1
786-171
SpinOUT™ GT-600, 3ml
10 3
0.1-0.5
786-704
SpinOUT™ GT-600, 5ml
5 5
0.5-2
786-705
SpinOUT™ GT-600, 10ml
5 10
0.5-4
786-706
SpinOUT™ GT-1200, 0.1ml
25 0.1
0.005-0.02
786-1821
SpinOUT™ GT-1200, 0.5ml
10 0.5
0.025-0.1
786-172
SpinOUT™ GT-1200, 1ml
10 1
0.05-0.1
786-1822
SpinOUT™ GT-1200, 2ml
10 2
0.1-0.2
786-173
SpinOUT™ GT-1200, 3ml
10 3
0.1-0.5
786-707
SpinOUT™ GT-1200, 5ml
5 5
0.5-2
786-708
SpinOUT™ GT-1200, 10ml
5 10
0.5-4

 

For the desalting and clean up of PCR reactions, click here.
 

 

 


Features

  • Offered in 3 MWCO sizes: >700Da,  >6kDa and >30kDa proteins 
  • Available in 5 sizes for sample volumes of 5µl to 4ml
  • 96-well plate formats available
  • Easy-to-use, spin-format columns for rapid purification

 


Applications

  • Protein purification and contamination removal from samples
  • For the desalting and buffer exchange of protein and nucleic acid solutions
  • Separate proteins from peptides
  • Desalt peptides 

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