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Immobilized Streptavidin Resin

Immobilized Streptavidin Resin
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Immobilized Streptavidin Resin
Immobilized Streptavidin Resin
Catalog Description Size Price (USD) Qty
786-390Streptavidin, Immobilized5ml resin$263.00
786-555Streptavidin Resin Kit1$676.00
786-590Streptavidin, Immobilized2ml resin$184.00
786-591Streptavidin, Immobilized10ml resin$410.00
786-592Streptavidin, Immobilized5 x 1ml$494.00
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  • Description
    Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M guanidine-hydrochloride and extreme levels of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at a pH of 1.5, or by boiling in SDS Page Sample Loading Buffer.
     

    Streptavidin is a tetrameric protein containing 4 biotin binding sites. In many respects, streptavidin is similar to avidin, but it does not contain a carbohydrate, and its molecular weight (about 60kDa) is slightly lower. The solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin; however, the binding of streptavidin to biotin is very similar to that of avidin.

     

    The advantage of streptavidin is that the lack of carbohydrates significantly reduces the amount of non-specific binding.

     

    The streptavidin used for immobilization on porous 6% cross-linked agarose is a recombinant form, with a mass of 53kDa and a near neutral pH. The streptavidin is covalently coupled to the agarose, resulting in minimal leaching, and is stable over a pH range of 2 to 11.

     

    The Streptavidin Resin is designed for the single-step, small and large scale affinity purification of proteins, antibodies and other molecules with a biotin tag. The resin can also be used for assay development and immunopreciptations of proteins using biotin labeled antibodies.

     

    Our streptavidin is supplied as resin slurry or in a 1ml spin-column format.

     

    Specific Binding and Elution Buffers are also available.

     

    Click here to request bulk or custom sizes.

     

     


    Features

    • Recombinant streptavidin covalently coupled to ~6% cross-linked agarose for minimal leaching
    • Single-step, small & large scale affinity purification
    • Supplied as resin slurry or in spin-column format
    • Binding Capacity: 15-30 µg biotin/ml resin
    • Ligand Density: > 1 mg biotin-BSA/ml resin
    • Stability Range: pH2-11

     


    Applications

    • Immunopreciptation of proteins using biotin labeled antibodies
    • Assay development (pull down assays) using biotinylated proteins
    • Purification of biotinylated molecules, including: proteins, antibodies, DNA, carbohydrates & other molecules with a biotin tag

     

     

  • Reviews
Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M guanidine-hydrochloride and extreme levels of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at a pH of 1.5, or by boiling in SDS Page Sample Loading Buffer.
 

Streptavidin is a tetrameric protein containing 4 biotin binding sites. In many respects, streptavidin is similar to avidin, but it does not contain a carbohydrate, and its molecular weight (about 60kDa) is slightly lower. The solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin; however, the binding of streptavidin to biotin is very similar to that of avidin.

 

The advantage of streptavidin is that the lack of carbohydrates significantly reduces the amount of non-specific binding.

 

The streptavidin used for immobilization on porous 6% cross-linked agarose is a recombinant form, with a mass of 53kDa and a near neutral pH. The streptavidin is covalently coupled to the agarose, resulting in minimal leaching, and is stable over a pH range of 2 to 11.

 

The Streptavidin Resin is designed for the single-step, small and large scale affinity purification of proteins, antibodies and other molecules with a biotin tag. The resin can also be used for assay development and immunopreciptations of proteins using biotin labeled antibodies.

 

Our streptavidin is supplied as resin slurry or in a 1ml spin-column format.

 

Specific Binding and Elution Buffers are also available.

 

Click here to request bulk or custom sizes.

 

 


Features

  • Recombinant streptavidin covalently coupled to ~6% cross-linked agarose for minimal leaching
  • Single-step, small & large scale affinity purification
  • Supplied as resin slurry or in spin-column format
  • Binding Capacity: 15-30 µg biotin/ml resin
  • Ligand Density: > 1 mg biotin-BSA/ml resin
  • Stability Range: pH2-11

 


Applications

  • Immunopreciptation of proteins using biotin labeled antibodies
  • Assay development (pull down assays) using biotinylated proteins
  • Purification of biotinylated molecules, including: proteins, antibodies, DNA, carbohydrates & other molecules with a biotin tag

 

 

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