SG-Lysine-C™

SG-Lysine-C^™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C^™ are chemically modified making the enzyme resistant to autolysis and stabilizing its enzymatic activity.

SG-Lysine-C^™ is supplied lyophilized in 20μg vials. The enzyme is typically reconstituted to a concentration of 0.25μg/ml. For fragmentation, the enzyme is added to the sample protein in a ratio of 1:100 to 1:20 (enzyme to protein, by weight) in a standard digestion buffer.

Features

• Modified Lysine-C for sequence analysis.

• Resistant to autolysis and degradation.

• High Specificity, free from other known endopeptidases.

• Consistency of activity levels from lot to lot for reproducible digestions.

• Recommended SG-Lysine-C™ to protein ratio, 1:100 to 1:20.

Applications

• Digestion of proteins for sequence analysis.

• Suitable for sequencing applications.